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PKH67GL

Sigma-Aldrich

PKH67 Green Fluorescent Cell Linker Kit，用于常规细胞膜标记

Distributed for Phanos Technologies

别名:

Green PKH membrane labeling kit

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1 KIT
$1,320.00

$1,320.00

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1 KIT
$1,320.00

About This Item

UNSPSC代码:
12352207
NACRES:
NA.32

$1,320.00

请联系客服了解存货情况

质量水平

200

包装

pkg of 1 kit

储存条件

protect from light

荧光

λex 490 nm; λem 502 nm (PKH67 dye)

检测方法

fluorometric

运输

ambient

储存温度

room temp

相关类别

应用

本试剂盒适用于常规细胞膜标记。PKH67具有长于PKH1和PKH2的脂肪炭尾，后两者是另外两种之前常用于体外和体内细胞示踪的绿色染料。由于炭尾长度更长，内部研究已经证明PKH67比PKH2由更少的细胞间转移。
在采用PKH1和PKH2进行的体内研究中，荧光强度都会缓慢损失。由于这是绿色细胞linker染料而非红色细胞linker染料出现的行为特征，因而PKH67会出现类似的性质。不分裂细胞的体外细胞膜留存和体内荧光半衰期的关联性揭示，PKH67的体内荧光半衰期为10-12天。其他具有类似半衰期的绿色细胞linker染料已经被用于监测1-2月内的体内 淋巴细胞和巨噬细胞运输，结果表明PKH67还可用于中等时长的体内跟踪研究。
用于常规细胞膜标记的PKH26红色荧光细胞连接子试剂盒已用于：
  • 标记并进一步研究在三阴性乳腺癌状况下从细胞中排出的外泌体。[1]
  • 标记凋亡性细胞，[2]用于研究ανβ5受体在凋亡性细胞的结合和内化中的作用。[3]
  • 在荧光成像中。[4]

联系

如需关于PKH及CellVue®的荧光细胞连接子染料的更多技术详情，包括大量的参考书目，请访问这里。

法律信息

CellVue is a registered trademark of Phanos Technologies

仅试剂盒组分

产品编号
说明
  • Diluent C 6 x 10
  • PKH67 Cell Linker in ethanol .5 mL

象形图

FlameExclamation mark
GHS02,GHS07

警示用语：

Danger

危险声明

H225,H319

危险分类

Eye Irrit. 2 - Flam. Liq. 2

储存分类代码

3 - Flammable liquids

闪点(°F)

57.2 °F - closed cup

闪点(°C)

14 °C - closed cup

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCV4102
MKCQ9079
MKCP3216
MKCQ0194
MKCK9600

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Exosomes from triple-negative breast cancer cells can transfer phenotypic traits representing their cells of origin to secondary cells
O Brien K, et al.
European Journal of Cancer, 49(8), 1845-1859 (2013)
Anjali P Kusumbe et al.
Stem cells (Dayton, Ohio), 27(3), 498-508 (2009-03-04)
Recruitment and localization of endothelial precursors within tumors is a potential area for the development of therapeutics, because their functional contribution to tumor vasculature is realized to be important for cancer cell survival. However, the exact nature of the recruited
Hybrid microscaffold-based 3D bioprinting of multi-cellular constructs with high compressive strength: A new biofabrication strategy
Yu Jun T, et al.
Scientific Reports, 6, 39140-39140 (2016)
Dandan Ma et al.
Cells, 9(4) (2020-04-01)
Human salivary histatin 1 (Hst1) and Hst2 exhibit a series of cell-activating properties (e.g., promoting adhesion, spreading, migration and metabolic activity of mammalian cells). In contrast, Hst5 shows an anti-fungal property but no cell-activating properties. Previous findings suggest that their
alpha v beta 5 integrin recruits the CrkII-Dock180-Rac1 complex for phagocytosis of apoptotic cells
Albert, Matthew L and Kim, Jong-Ii and Birge, Raymond B
Nature Cell Biology, 2(12), 899-899 (2000)
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PKH染料易于使用，可对活细胞进行稳定、均匀和可再现的荧光标记。PKH染料是无毒的膜染色剂，能产生高信噪比。

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用于细胞追踪的亲脂性染料试剂盒

亲脂性细胞追踪染料使癌症生物学家能够在体外和体内追踪肿瘤和免疫细胞功能。阅读文章，选择适合细胞追踪和增殖监测的膜染料试剂盒。

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Questions

1–10 of 12 Questions  

  1. I mark mitochondria for injection with PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling. I want to use another dye to mark another type of mitochondria and inject them together in the same fish egg. Is PKH67 ok for this like PKH26?

    1 answer

    1. Yes, Product PKH67 would be suitable for this application. In this paper, both Products PKH26 and PKH67 are used in a similar application:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5679712/

      Helpful?

  2. What can be the alternative for removing excess dye after labeling of exosomes using PKH67 dye apart from ultracentrifugation?

    1 answer

    1. Excess dye is bound to serum or albumin which is added to the cell/dye mixture as a stop reaction. The recommended clean up or dye removal is by standard centrifugation (400 x g for 10 minutes, wash, and repeat). See the Product Information Sheet, page 3, bullet point 8 for more information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/984/984/pkh67glbul.pdf

      Alternate methods for excess dye removal have not been investigated.

      Helpful?

  3. Can we remove the excess PKH67 DYE used for the labeling of exosomes using exosome spin column rather than ultracentrifugation.

    1 answer

    1. The recommended exosome labeling protocol has been optimized to assure the best possible results. The use of exosome spin columns with the PKH and CellVue products has not been validated. See below for a link to our exosome labeling protocol. A spin column method may not offer the best results.

      https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/imaging-analysis-and-live-cell-imaging/exosome-labeling-pkh

      Helpful?

  4. Will Product PKH67, Green Fluorescent Cell Linker Kit, stain dead cells?

    1 answer

    1. As long as the cell has an intact membrane, the PKH76 dye can label the cell.

      Helpful?

  5. What is the difference between Green Fluorescent Cell Linker Kits PKH2 and PKH67?

    1 answer

    1. PKH2 was one of the early PKH dyes.  The PKH67 dye has a longer aliphatic tail.  There is reduced cell-celldye transfer for PKH67 as compared with PKH2.

      Helpful?

  6. What method of fixation can be used for tissue/cells with the PKH Fluorescent Cell Linker Kit for General Cell Membrane Labeling dyes?

    1 answer

    1. A protocol for visualization of tissue sections can be found in the technical bulletin.  For visualization of stained cells by immunofluorescence or flow cytometry, the cells can be fixed in 2% paraformaldehyde for 15 minutes. The use of other organic solvents will extract the dye from the cells.  If internal labeling is desired, the cells can be permeabilized with saponin (50-75 μg/mL). Here is the link to the Troubleshooting Guide.

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. What is the excitation and emission spectra for Product PKH67GL, Green Fluorescent Cell Linker Kit?

    1 answer

    1. The spectra can be found on the product insert.The product has a maximum excitation at 490 nm and maximum emission at 502 nm.

      Helpful?

  9. How many cells can be stained with Product PKH67GL, Green Fluorescent Cell Linker Kit?

    1 answer

    1. This kit can stain 50 × 107 cells if used as directed (1 × 107 cells stained with 2 × 10-6 M PKH67).

      Helpful?

  10. When using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling, how long will the cells remain stained after treatment?

    1 answer

    1. Labeled cells that have been washed can be visualized in culture up to 100 days after staining (for non-dividing cells). The dye itself is stable and will divide equally when the cells divide. After staining with PKH dyes, you can observe as many as 8 divisions depending on how brightly the cells were stained initially and the amount of surface area on the cells. Most commonly, 4-6 divisions can be visualized.

      Helpful?

1–10 of 12 Questions  

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