The PKH cell linker kits have not been specifically tested for the detection of circulatory fibrocytes. However, there are several publications that indicate suitability. Please see the link below to review one such reference:
https://onlinelibrary.wiley.com/doi/full/10.1111/jcmm.13080
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应用
该试剂盒用于标记吞噬细胞。可以选择性标记具有吞噬能力的细胞，例如单核细胞、巨噬细胞或中性粒细胞。
原理
吞噬细胞的具体标记方法是形成染料聚集体或颗粒物。这样可以显著抑制淋巴细胞等非吞噬细胞对染料的吸收，同时促进吞噬细胞对染料的吸收。因为染料聚集于这些细胞的吞噬小体内，标记后的细胞呈片状或点状色斑。这些染料不易被细胞代谢清除，可在体内保持至少21天。
这种方法标记吞噬细胞可同时在体外或体内进行。通过腹膜内或静脉内注射PKH26标记溶液来完成可完成体内标记，而从体内分离出来的靶标细胞则可通过体外 标记方法进行染色。
这种方法标记吞噬细胞可同时在体外或体内进行。通过腹膜内或静脉内注射PKH26标记溶液来完成可完成体内标记，而从体内分离出来的靶标细胞则可通过体外 标记方法进行染色。
联系
有关PKH Fluorescent Cell Linker Dyes的其他技术信息，包括完整的参考书目，请访问这里。
法律信息
CellVue is a registered trademark of Phanos Technologies
仅试剂盒组分
产品编号
说明
- Diluent B 6 x 10
- PKH26 cell linker in ethanol .5 mL
警示用语：
Danger
危险声明
危险分类
Eye Irrit. 2 - Flam. Liq. 2
储存分类代码
3 - Flammable liquids
闪点(°F)
57.2 °F - closed cup
闪点(°C)
14.0 °C - closed cup
其他客户在看
Jeffrey H Jennings et al.
American journal of respiratory cell and molecular biology, 32(2), 108-117 (2004-11-26)
Apoptotic cells must be cleared to resolve inflammation, but few resident alveolar macrophages (AMo) from normal lungs ingest apoptotic cells. We examined how Mo ingestion of apoptotic cells is altered during immune inflammation induced by intratracheal challenge of primed C57BL/6
Hansang Cho et al.
Lab on a chip, 14(5), 972-978 (2014-01-17)
Neutrophils are the most abundant type of white blood cells in the circulation, protecting the body against pathogens and responding early to inflammation. Although we understand how neutrophils respond to individual stimuli, we know less about how they prioritize between
Hiroshi Kanno et al.
International journal of molecular sciences, 14(5), 9604-9617 (2013-05-07)
A specialized population of cells residing in the hair follicle is quiescent but shows pluripotency for differentiating into epithelial-mesenchymal lineage cells. Therefore, such cells are hoped to be useful as implantable donor cells for regenerative therapy. Recently, it was reported
Solène Accarias et al.
Innate immunity, 22(5), 382-392 (2016-05-26)
Resident macrophages play a central role in maintaining tissue homeostasis and immune surveillance. Here, we used single cell-based qPCR coupled with flow cytometry analysis to further define the phenotypes of large and small resident peritoneal macrophages (LPMs and SPMs, respectively)
U Maus et al.
American journal of physiology. Lung cellular and molecular physiology, 280(1), L58-L68 (2001-01-03)
The evaluation of monocytes recruited into the alveolar space under both physiological and inflammatory conditions is hampered by difficulties in discriminating these cells from resident alveolar macrophages (rAMs). Using the intravenous injected fluorescent dye PKH26, which accumulated in rAMs without
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Is this kit suitable for label circulatory fibrocyte isolated from rat PBMC?
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