One reference gives a pI of 9.4 for subtilisin Carlsberg. See: Zacharis, E. et al., Proc. Nat. Acad. Sci. USA, 96, 1201-1205 (1999).
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类型
Type XXIV
表单
lyophilized powder
比活
≤0.05 Kunitz units/mg solid RNase
≤5.0 Kunitz units/mg solid DNase
7.0-14.0 units/mg solid
分子量
27 kDa
纯化方式
crystallization
运输
wet ice
储存温度
−20°C
相关类别
特异性
枯草杆菌蛋白酶A是丝氨酸S8内蛋白酶家族的一员。它具有宽泛的特异性并在P1位对大的未带点残基具有偏好性。它可水解天然和变性的蛋白，并在碱性条件下具有活性。
应用
这是从地衣芽孢杆菌发酵中分离得到的蛋白水解酶。它是一种对天然和变性蛋白具有广泛特异性的丝氨酸内切蛋白酶，并在碱性条件下具有活性。 产品P8038，也被称为枯草杆菌蛋白酶Carlsberg，已用于水解心肌细胞以研究心脏线粒体NHE1的沉默[3]。
生化/生理作用
蛋白酶可通过肽键的水解使蛋白质分解代谢。 蛋白酶可被丝氨酸活性位点抑制剂所灭活，如苯甲基磺酰氟（PMSF）和二异丙基氟磷酸盐。
物理属性
枯草杆菌蛋白酶是一种具有27 KDa分子量并不含有二硫键的非糖基化单一多肽链。
单位定义
在pH 7.5，37 °C 条件下，一单位的酶每分钟将会水解酪蛋白以产生与1.0 μ摩尔 (181 μg) 酪氨酸等同的颜色（Folin-Ciocalteu试剂提供的颜色）。
分析说明
氨基酸分析和等电聚焦电泳与枯草杆菌蛋白酶Carlsberg一致。
警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
10 - Combustible liquids
WGK
WGK 2
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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What is the pI of Product P8038, Proteinase, bacterial?
1 answer-
Helpful?
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What is the pH optimum for Product P8038, Proteinase, bacterial?
1 answer-
Two references cite pH 7.5 as optimal for this enzyme: (a) Ricks et al., Biotechnol. Prog., 8, 197-203 (1992), (b) Doswald et al., Bioorg. Med. Chem., 2, 403-410 (1994).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight (MW) of Product P8038, Proteinase, bacterial?
1 answer-
The enzyme has a MW of 27.5-27.6 kDa. See DeLange & Smith, J. Biol. Chem., 243(9), 2134-2142 (1968).
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How long can I store stock solutions of Product P8038, Proteinase, bacterial?
1 answer-
Our notes indicate that a 0.1% solution (1 mg/mL) in PBS or TBS can be stored for about a week at 0-4°C. For longer term storage, we would advise freezing the stock solution in aliquots.
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