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應用
在分离DNA和RNA的过程中，用于核酸酶的蛋白水解失活。
用于去除内毒素结合的阳离子蛋白，如溶菌酶和核糖核酸酶A。
据报道，其可用于分离肝、酵母和绿豆线粒体
用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理，暴露用于抗体标记的抗原结合位点。
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。
用于去除内毒素结合的阳离子蛋白，如溶菌酶和核糖核酸酶A。
据报道，其可用于分离肝、酵母和绿豆线粒体
用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理，暴露用于抗体标记的抗原结合位点。
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。
生化／生理作用
蛋白酶K是一种稳定且具有高活性的丝氨酸蛋白酶。晶体和分子结构研究证据表明该酶属于枯草素家族，具有活性位点催化三联体(Asp39-His69-Ser224)。它在一系列广泛的环境中均稳定：pH，缓冲盐，去污剂（SDS）以及温度。 在0.1-0.5% SDS存在的情况下，蛋白酶K可保持活性，在不损害分离DNA完整性的前提下，可以消化DNA制剂中的多种蛋白质和核酸酶。
分析報告
外观（颜色）：白色
外观（描述）：冻干粉末
活性（血红蛋白； pH 7.5；37 °C）： ≥ 30.0 mAnsonU/mg
比活力（以蛋白质计算）： ≥ 40 mAnsonU/mg
脱氧核糖核酸酶（切口活性；pBR 322；6 H；37 °C）：未检出
RNA酶（RNA；2 h；37°C）：未检出
外观（描述）：冻干粉末
活性（血红蛋白； pH 7.5；37 °C）： ≥ 30.0 mAnsonU/mg
比活力（以蛋白质计算）： ≥ 40 mAnsonU/mg
脱氧核糖核酸酶（切口活性；pBR 322；6 H；37 °C）：未检出
RNA酶（RNA；2 h；37°C）：未检出
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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商品
Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.
Pro K aids in disrupting cell membranes for DNA release, crucial for downstream molecular biology techniques.
Guidelines on use of proteinase K, an enzyme commonly used to degrade proteins, and protect DNA and RNA from degradation in samples.
Balancing Proteinase K cost with quality and technical support ensures optimal enzyme selection for diverse applications.
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