The solution stability of this product has not been determined. However, other collagenase products available have been determined to be stable when stored in solution at –20°C. Repeated freeze/thaw cycles are not recommended and may result in a decline in activity. Please see the link below to review the product datasheet for C2139, a collagenase preparation for the isolation of adipocytes and hepatocytes:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/173/127/c2139pis.pdf
推荐产品
一般說明
II型胶原酶(Cls II)是溶组织梭菌分泌的主要的一种组织解离酶。它帮助消化I型胶原酶分解而成的胶原片段。胶原酶属于基质金属蛋白酶(MMP)家族成员，消化细胞外基质(ECM)中的胶原。胶原蛋白是由三条胶原多肽链以高拉伸强度缠绕而成的三螺旋结构蛋白。[1]
该产品与Biochrom GmbH部件号C2-22和C2-28相同。由胶原酶、梭菌蛋白酶混合而成的酶，具有来自溶组织梭菌的胰蛋白酶和蛋白水解活性。该制剂显示高梭菌蛋白酶活性，其胰蛋白酶活性接近于I型。适用于肝脏，骨骼，甲状腺，心脏和唾液腺组织。比活度为每毫克粉末物质125至250 Mandl单位。
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Tissue dissociation enzymes for adipose stromal vascular fraction cell isolation: a review
Lockhart RA, et al.
Journal of stem cell research & therapy (2015)
Yu-Yao Zhang et al.
Stem cell research & therapy, 13(1), 340-340 (2022-07-27)
Nucleus pulposus mesenchymal stem cells (NPMSCs) transplantation is a promising treatment for intervertebral disc degeneration (IVDD). However, the transplanted NPMSCs exhibited weak cell proliferation, high cell apoptosis, and a low ability to resist the harsh microenvironment of the degenerated intervertebral
Integrin αVβ3 Signaling in the Progression of Osteoarthritis Induced by Excessive Mechanical Stress.
Fanglong Song et al.
Inflammation, 46(2), 739-751 (2022-12-09)
Osteoarthritis (OA) is believed to be linked with cartilage degeneration, subchondral bone sclerosis, and synovial inflammation that lead to joint failure, and yet treatment that can effectively reverse the pathological process of the disease still not exists. Recent evidence suggests
Pei-Long Liu et al.
Analytical cellular pathology (Amsterdam), 2023, 7573165-7573165 (2023-05-18)
Diabetic neuropathic osteoarthropathy (DNOAP) is a rare and easily missed complication for diabetes that leads to increased morbidity and mortality. DNOAP is characterized by progressive destruction of bone and joint, but its pathogenesis remains elusive. We herein aimed to investigate
Yuan Yang et al.
BMC pharmacology & toxicology, 23(1), 16-16 (2022-03-23)
Arsenic, existing ubiquitously in soil, drinking water, or food, is well known to be an environmental pollutants concerned by European Food Safety Authority. Lentinan, a beta-1,6;1,3-glucan extracts from Lentinus edodes, which has the properties of antioxidant and immunomodulation, present study
-
Dear Sir, I stored reconstituted collagenase in a -20 degrees fridge after diluting and using some of it, after 4months is the enzyme still viable for use? It was left in -20 degrees all the while, thanks
1 answer-
Helpful?
-
-
Kann die Kollagenase für die enzymatische Verdauung und Isolierung von Hühnerembryoosteoblasten verwendet werden? Wie ist die Reinheit des Produkts?
1 answer-
This product has not been tested for use in isolating osteoblasts from chick embryos. Currently, there is no collagenase product option that has been tested for suitability in this application. The product is generally used for bone tissue digests. However the end user would have to determine suitability experimentally.
A preliminary search of PubMed and Google Scholar did not find research articles citing this specific application. The working enzyme concentration range is typically 0.1 to 0.2% (w/v), depending on the delicate nature of the tissue substrate. This product is tested for enzymatic activity. The analytical purity has not been determined.
Helpful?
-
-
골관절염 모델링에 있어 collagenase type 2 를 500 U로 Intra-articular injection 해야합니다만, spec sheet 에 쓰여있는 바로는 units/mg Solid (Collagen) 이 >= 125 로 되어있는데 mg 당 정확한 unit을 알수는 없나요 ?
1 answer-
The exact value is reported on the lot specific Certificate of Analysis. Please see the link below to access a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sial/c2bioc#product-documentationHelpful?
-
-
Dear sir I was bought collagenase before, but I can't find my instruction manual. Can I have a copy? I need to prepare 0.2% solution to dissolve tumor cells, and I want to grow solute for greenhouse configuration and two for dissolving cells.
1 answer-
Unfortunately, no user manual is provided with this product. It is recommended to prepare working solution by dissolving the enzyme in water to a final concentration of 100mg/mL. Dilute with PBS (Ca2+/Mg2+-free) to a final concentration of 1mg/mL and filter through a sterile 0.2μm membrane filter.
Another Collagenase Type II product under the Sigma-Aldrich brand, C6885, does offer a product information sheet. While the lot-specific activity between the two products varies, the storage, handling, and functional information would be the same. See the link below to review this document:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/321/500/c6885pis.pdfHelpful?
-
Active Filters
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门
