推荐产品
product name
聚-D-赖氨酸 氢溴酸盐, mol wt 30,000-70,000
形狀
solid
分子量
30,000-70,000
技術
cell culture | mammalian: suitable
顏色
white to off-white
儲存溫度
−20°C
SMILES 字串
O=C(C)[C@@](NC)([H])CCCCN.[Br]
InChI
1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H
InChI 密鑰
MEXAGTSTSPYCEP-UHFFFAOYSA-N
正在寻找类似产品? 访问 产品对比指南
應用
聚-D-赖氨酸聚合物可用于制备细胞附着的表面。D-赖氨酸聚合物也可用于消化聚-L-赖氨酸聚合物并导致L-赖氨酸过量摄取的细胞。
建议使用0.5 - 1.0 mL的该产品0.1 mg/mL溶液用作细胞培养的基质对25 cm2进行涂覆。 较低分子量的该产品粘度相对较低，但较高分子量的形式则可提供每分子更多的附着位点。
建议使用0.5 - 1.0 mL的该产品0.1 mg/mL溶液用作细胞培养的基质对25 cm2进行涂覆。 较低分子量的该产品粘度相对较低，但较高分子量的形式则可提供每分子更多的附着位点。
生化／生理作用
多聚-L-赖氨酸（PDL）氢溴酸盐是细胞的非特异性粘附因子，它通过增强细胞膜表面的负电荷离子与培养表面之间的静电相互作用，促进细胞与固相基质的粘附。 当被细胞培养表面吸收后，聚-D-赖氨酸可增加带正电的细胞结合位点数量。
成分
聚-D-赖氨酸是一种带正电的氨基酸聚合物，其中每个赖氨酸残基约有一个HBr。 氢溴酸盐可使聚-D-赖氨酸呈可溶于水的结晶形式。 在β结构中可能会发现少量的产物，因为HBr干扰氨基与羧基或含N或O部分之间的氢键。
注意
无菌溶液可在2-8℃条件下稳定保存2年。脱水保存在-20°C条件下。
準備報告
为了除去HBr，可将该产品溶解在中性缓冲液中并透析以除去盐。 一般来说，要将此产品用作附着因子，可将50 mL无菌组织培养级水加入至5 mg聚赖氨酸中，并按每25cm2 1 mL的比例将溶液无菌涂抹在表面上。 5分钟后，通过抽吸除去溶液并彻底冲洗表面。 在引入细胞和培养基之前让其干燥两小时。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Mandi E Schmidt et al.
BMC biology, 16(1), 58-58 (2018-06-28)
Huntington disease (HD) is a fatal neurodegenerative disorder caused by a CAG expansion in the huntingtin (HTT) gene, leading to selective and progressive neuronal death predominantly in the striatum. Mutant HTT expression causes dysfunctional cortico-striatal (CS) transmission, loss of CS
Louise Gorham et al.
European journal of pharmacology, 736, 101-106 (2014-04-29)
Somatostatin has a wide biological profile resulting from its actions on the five receptor subtypes (sst1-5). Recently somatostatin was shown to exert analgesic effects via activation of the sst4 receptor. Although the analgesia in pain models is established, the precise
Michael Brunk et al.
Scientific reports, 8(1), 605-605 (2018-01-14)
The dynamics of early fungal development and its interference with physiological signals and environmental factors is yet poorly understood. Especially computational analysis tools for the evaluation of the process of early spore germination and germ tube formation are still lacking.
William J Buchser et al.
Molecular systems biology, 6, 391-391 (2010-07-29)
Development and regeneration of the nervous system requires the precise formation of axons and dendrites. Kinases and phosphatases are pervasive regulators of cellular function and have been implicated in controlling axodendritic development and regeneration. We undertook a gain-of-function analysis to
Nobuyuki Futai et al.
Micromachines, 10(1) (2018-12-28)
We have developed a cast microfluidic chip for concentration gradient generation that contains a thin (~5 µm² cross-sectional area) microchannel. The diffusion of diffused 185 nm ultraviolet (UV) light from an inexpensive low-pressure mercury lamp exposed a layer of the
商品
Humankind has utilized protein materials throughout its existence, starting with the use of materials such as wool and silk for warmth and protection from the elements and continuing with the use of recombinant DNA techniques to synthesize proteins with unique and useful properties.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门