This product has not been tested in-house for the application of assessing the effect of enzymes in pancreatin on fungus and fungal cell walls, specifically with lipase. A recommended concentration has not been established. Conducting a literature search may provide guidance on this matter.
推荐产品
应用
生化/生理作用
由于胰蛋白酶、淀粉酶和脂肪酶等酶的作用，胰酶可水解蛋白质、淀粉和脂肪。 胰酶能在40°C的水中在5分钟内将不少于其25倍重量的土豆淀粉转化为可溶性碳水化合物，可在40℃，pH7.5条件下，在60分钟内消化不少于自身重量25倍的酪蛋白并可在37℃，pH9.0条件下，从橄榄油中每分钟每毫克胰酶释放不少于2微当量的酸。
胰酶由猪胰腺的外分泌细胞产生，含有胰蛋白酶、淀粉酶和脂肪酶、核糖核酸酶和蛋白酶等酶组份。 这种酶的组合使其能够水解蛋白质、淀粉和脂肪。 在 40°C 水中，胰酶在 5 分钟内将不少于其重量 25 倍的马铃薯淀粉转化为可溶性碳水化合物，在 pH7.5，40℃ 下，胰酶在 60 分钟内消化不少于重量 25 倍的酪蛋白，在 pH9.0，37℃ 下，每毫克胰酶每分钟将从橄榄油中释放不低于微当量的酸。
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 1
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Estimation of ruminal degradation and intestinal digestion of tropical protein resources using the nylon bag technique and the three-step in vitro procedure in dairy cattle on rice straw diets.
Promkot, C., M. Wanapat, and P. Rowlinson
Asian-Australasian Journal of Animal Sciences, 20(12), 1849-1849 (2007)
Yan Li et al.
Food chemistry, 221, 1151-1157 (2016-12-17)
Homologous and heterologous cross-linked polymers of whey protein isolate (WPI), soy protein isolate (SPI) and casein (CN) and their binary mixtures, viz., WPI+SPI, WPI+CN and SPI+CN, were produced using transglutaminase, and their in vitro IgE reactivity and digestibility under simulated
John A Monro et al.
The British journal of nutrition, 103(2), 295-305 (2009-11-26)
Glycaemic responses to foods reflect the balance between glucose loading into, and its clearance from, the blood. Current in vitro methods for glycaemic analysis do not take into account the key role of glucose disposal. The present study aimed to
Estelle J A Suys et al.
Journal of pharmaceutical sciences, 108(1), 193-204 (2018-12-07)
Polyethoxylated, nonionic surfactants are important constituents of many drug formulations, including lipid-based formulations. In an effort to better understand the behavior of formulation excipients at the molecular level, we have developed molecular dynamics (MD) models for the widely used surfactant
Yu Tian et al.
Foods (Basel, Switzerland), 9(3) (2020-03-15)
Noodles are widely consumed in China, which can be cooked in different ways. The effects of different cooking methods (boiling, steaming, microwave heating, stir-frying and frying) on the resistance starch (RS) content and digestive properties (digestion rate, digestibility and estimated
-
Hello, I want to assess the effect of enzymes in pancreatin on fungus and fungal cell wall specially (with lipase), is there a recommended concentration to use for pancreatin solution? and what is the recommended dissolving solution in this case? Thanks
1 answer-
Helpful?
-
-
Excuse me, is it possible to provide protease activity report for buyer? thank you very much and look forward to your reply.
1 answer-
This product meets 8X the minimum USP specifications. According to the USP monograph for Pancreatin, the 1X minimum specification is 25 USP units amylase, 2 USP units lipase, and 25 USP units protease per milligram. The calculated protease activity for the 8X preparation then, would be at least 200 units per milligram. This is a calculated value based on the USP monograph and are not reported separately on the Certificate of Analysis.
Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/p7545#product-documentationHelpful?
-
-
Hi, what is the dissovled soultion? It can't dissolved in water or 1 mM HCl
1 answer-
This product is not tested for solubility. For assay purposes. it is dissolved at 40 mg/3mL PBS, pH 6.8. Trituration is required for 5 - 10 minutes. Historically, this product has also been dissolved in water at 20 mg/ml. with heating at 37 °C. This method yields a hazy solution.
Helpful?
-
-
Is the product used for digestion cells in clinical laboratory? Can you please help me?
1 answer-
This product is for research use only and is not suitable for in vitro diagnostic studies.
Helpful?
-
-
What is the solubility of this enzyme in water?
1 answer-
This product is soluble in water at up to 20 mg/ml in water. Heat may be required. This concentration will yield a hazy solution.
Helpful?
-
-
What is the shelf life for this product?
1 answer-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
Helpful?
-
-
Bonjour, Que signifie exactement : '8 x SP specifications' ? Merci pour votre réponse
1 answer-
The specific activity of this pancreatin is specified to be at least 8 x the minimum USP standard.
The USP defines a minimum specification of a 1 X Pancreatin as follows:
- 25 USP protease units per mg.
- 25 USP amylase units per mg.
- 2 USP lipase units per mg.Therefore, activity will be 8 times that.
Helpful?
-
Active Filters
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门
