As mentioned in the 'DESCRIPTION' section, this product is a lyophilized powder containing (NH4)2SO4, sodium citrate and sodium chloride. This product is not tested for purity. However, buffer salts are expected to be the bulk of the non-protein content.
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種類
Type XIII-A
品質等級
形狀
lyophilized powder
比活性
300-1,500 units/mg protein
成份
Protein, 5-15% biuret
環保替代產品特色
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
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UniProt登錄號
環保替代類別
儲存溫度
−20°C
基因資訊
human ... AMY1A(276)
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一般說明
人α-淀粉酶由包含496个氨基酸的单个多肽链组成，其在1号染色体上进行编码。其在唾液腺或胰腺中产生。唾液和胰腺α-淀粉酶具有同源的一级序列，但表现出不同的切割模式。[1]
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。当用于淀粉乙醇研究时，该产品经优化可提高能效和防止浪费。有关详细信息，请参阅 《Biofiles》的文章。
應用
生化／生理作用
α-淀粉酶可水解三个或更多个D-葡萄糖单元通过α-（1,4）连接的多糖中的α-（1,4）葡聚糖键。天然底物（如淀粉和糖原）可被分解成葡萄糖和麦芽糖。[4]
單位定義
在pH 6.9、温度20 °C条件下，一个单位可在3分钟内从淀粉中释放出1.0 mg麦芽糖。
外觀
含有(NH4)2SO4、柠檬酸钠和氯化钠的冻干粉。
其他說明
查看更多关于 复合糖分析用酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
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Digestive enzyme inhibitors from grains as potential components of nutraceuticals.
D Piasecka-Kwiatkowska, et al.
J. Nutr. Sci. Vitaminol., 58, 217-220 (2012)
G D Brayer et al.
Protein science : a publication of the Protein Society, 4(9), 1730-1742 (1995-09-01)
The structure of human pancreatic alpha-amylase has been determined to 1.8 A resolution using X-ray diffraction techniques. This enzyme is found to be composed of three structural domains. The largest is Domain A (residues 1-99, 169-404), which forms a central
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实验方案
可根据此操作规程测定α-淀粉酶的活性。该α-淀粉酶酶促测定将会指导您完成整个过程及必要的计算。
Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.
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Hi, could you let me know what the remaining composition is when the product contains 5-15% protein? Thank you!
1 answer-
Helpful?
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Please let me know the storage temperature and storage period when it is dissolved in water
1 answer-
The stability of this material in solution has not been investigated. However, various sources suggest that solutions may be stored in working aliquots at -20°C for several months.
Helpful?
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What is the recommended method for dissolving alpha amylase obtained from human saliva, specifically A1031?
1 answer-
The recommended method is to dissolve the enzyme in 1mM calcium chloride (CaCl2).
Helpful?
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Could you please tell me the weight (mg or gram) of sample A1031?
1 answer-
This product is sold based on units of activity. The activity specification is 300 - 1500 U/mg. The unit per milligram value is reported in the lot-specific Certificate of Analysis and will range from 0.67 to 3.3 mg for the 1000 U pack size.
See the link below to review a sample Certificate:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A1031-1KU0000270112.pdfHelpful?
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