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Merck

P4809

Sigma-Aldrich

磷酸盐-柠檬酸盐缓冲液

tablet

别名:

PCR缓冲液

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50 TABS
$196.00
100 TABS
$314.00

$196.00


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50 TABS
$196.00
100 TABS
$314.00

About This Item

UNSPSC代码:
12161700
NACRES:
NA.25

$196.00


请联系客服了解存货情况

获取大包装报价

表单

tablet

pH值(酸碱度)

4.5-5.5 (1 tablet/100mL in water)

溶解性

water: soluble

储存温度

room temp

一般描述

磷酸柠檬酸缓冲液是一种常用的缓冲液,与可溶性辣根过氧化物酶底物一起使用,如邻苯二胺二盐酸(OPD)或四甲基联苯胺(TMB)。它的pH值为5.0,具有出色的缓冲能力。柠檬酸磷酸盐缓冲片可配制0.05 M、pH 5.0的柠檬酸磷酸盐缓冲液。这些片剂提供了一种快速、方便、准确的合成柠檬酸磷酸酯缓冲溶液的方法。它消除了在缓冲液制备中称重单个成分的耗时和繁琐的过程。[1]磷酸盐-柠檬酸缓冲片由最高质量的成分制造,以严格的物理和化学规格,以确保性能和批次间的一致性。pH 5.0的磷酸盐-柠檬酸盐缓冲液片剂已在免疫测定中配制为底物缓冲液。

应用


  • Characterization of nettle leaves (Urtica dioica) as a novel source of protease for clotting dromedary milk by non-destructive methods.:在酶促提取过程中使用磷酸-柠檬酸缓冲液保持植物来源酶的稳定性和活性,说明它在乳制品天然混凝剂开发中的重要作用(Bouazizi et al., 2022)。

  • A rapid, low pH, nutrient stress, assay to determine the bactericidal activity of compounds against non-replicating Mycobacterium tuberculosis.:使用磷酸-柠檬酸缓冲液,研究开发一种低pH营养应激检测方法,评估抗菌剂对结核休眠菌的杀菌疗效,提供重要药物开发工具(Early et al., 2019)。

  • Measurement of Free Iodine in Different Formulations of Povidone-Iodine Eye Drops 5.:用磷酸-柠檬酸缓冲液检测滴眼液中碘的稳定性和释放,说明它确保护眼产品疗效和安全性的重要作用(Prado et al., 2019)。

重悬

在25℃下,一片溶于100 mL去离子水中可得到0.05 M磷酸盐-柠檬酸盐缓冲液,pH 5.0。室温储存。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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In vivo experimental model of orthotopic dental pulp regeneration under the influence of photobiomodulation therapy.
Maria Stella Moreira et al.
Journal of photochemistry and photobiology. B, Biology, 166, 180-186 (2016-12-09)
Rose, N., et al.
Manual of Clinical Laboratory Immunology, 106-106 (1986)
Antonino Tuttolomondo et al.
Oncotarget, 8(37), 61415-61424 (2017-10-06)
Anderson-Fabry disease (AFD) is an inborn lysosomal enzymopathy resulting from the deficient or absent activity of the lysosomal exogalactohydrolase, α-galactosidase A. This deficiency, results in the altered metabolism of glycosphingolipids which leads to their accumulation in lysosomes, thus to cellular
P R Garrido et al.
Journal of photochemistry and photobiology. B, Biology, 194, 149-157 (2019-04-08)
Photobiomodulation therapy (PBMT) and the cell sheet (CS) technology improve processes relevant to tissue regeneration. The aim of this study was to investigate the effects of different PBMT parameters on the architecture (histology), protein composition (Western blotting and immunohistochemistry) and
Ross R Keller et al.
Carcinogenesis, 37(8), 810-816 (2016-05-22)
Carcinogen exposures inscribe mutation patterns on cancer genomes and sometimes bias the acquisition of driver mutations toward preferred oncogenes, potentially dictating sensitivity to targeted agents. Whether and how carcinogen-specific mutation patterns direct activation of preferred oncogenes remains poorly understood. Here

Questions

1–3 of 3 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Can this tablet be used for coagulation of. Milk for making cheese

    1 answer
    1. This product is intended primarily for laboratory research purposes unless otherwise stated. This product not to be used for any other purposes, including but not limited to, in vitro diagnostic purposes, in foods, drugs, medical devices or cosmetics for humans or animals or for commercial purposes.

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