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Merck
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P4417

Sigma-Aldrich

Phosphate buffered saline

Phosphate buffered saline
1 of 3 reviewers received a sample product or took part in a promotion

tablet, pH 7.2-7.6 (1 tablet/200 mL)

别名:

PBS

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50 TABS
$200.00
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$338.00

$200.00

预计发货时间2025年1月27日详情

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50 TABS
$200.00
100 TABS
$338.00

About This Item

MDL號碼:
MFCD00131855
分類程式碼代碼:
12161700
eCl@ss:
32129211
NACRES:
NA.25

$200.00

预计发货时间2025年1月27日详情

获取大包装报价

产品名称

Phosphate buffered saline, tablet

形狀

tablet

品質等級

200

儲存條件

dry at room temperature

顏色

white

pH值

7.2-7.6 (1 tablet/200 mL)

溶解度

water: soluble

密度

1.02 g/cm3 at 20—25 °C
1.03 g/cm3 at 20—25 °C

應用

diagnostic assay manufacturing
histology
life science and biopharma

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相关类别

一般說明

磷酸盐缓冲液（PBS）是一种在生物学研究中得到广泛应用的缓冲溶液，其多功能性和关键属性得到高度认可。特别值得一提的是，其等渗性与人体的pH、渗透压和离子浓度密切相关，它对细胞没有毒性。这种关键特性在多种细胞生物学实验中起到关键作用，包括细胞的温和清洗、组织的安全转运以及细胞和试剂的精确稀释。这些应用有助于保持细胞活力和构建有利的生理环境。PBS的缓冲能力来自于磷酸盐-磷酸的平衡，为实现最佳酶活性和各种生物学过程创建稳定的pH 环境。这种特性令PBS成为试剂制备、蛋白质透析和免疫组织化学等研究工作的理想选择,确保了实验结果的再现性和可靠性.

除了细胞生物学领域外，PBS的多功能性还体现在各种应用中，如为实现最佳抗体-抗原相互作用而构建定制化ELISA缓冲液。此外，它可用作蛋白A/G色谱的高效缓冲液，有助于基于特定抗体结合纯化抗体。在血液学和生物化学操作过程中，PBS 在细胞和组织的有效清洗中起关键作用。总之，PBS是生物学和生物化学研究中不可缺少的工具，具有等渗性、缓冲能力、简单和稳定的特性。其在细胞生物学、试剂制备、蛋白质纯化和各种生物分析中的多样化应用体现了其在增进科学知识和理解中的重要性。

應用

磷酸缓冲盐已用于-
  • 生产铂纳米颗粒(NP-Pt)水解胶体[1]
  • 在SEM之前清洗血液样品和SEM（扫描电子显微镜）存根[1]
  • 制备荧光标记小麦胚芽凝集素(WGA)溶液[2]
  • 在ELISA（酶联免疫吸附测定）期间稀释捕获抗体[3]
  • 在离心过程中悬浮细胞沉淀[3]
  • 免疫测定程序

特點和優勢

  • 适用于各种研究应用的优质产品
  • 可用作生物学和生物化学应用的缓冲液成分
  • 易溶于水

重構

将一片溶于200ml去离子水中，在25 °C下得到0.01m磷酸盐缓冲液、0.0027m氯化钾和0.137m氯化钠，pH值为7.4。

其他說明

如需了解生化试剂系列的更多信息，请填写此表。

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000373935
0000373935
0000362152
0000362150
0000362151

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Structural damage of chicken red blood cells exposed to platinum nanoparticles and cisplatin.
Kutwin M et al
Nanoscale Research Letters, 9(1), 257-257 (2014)
Lipid rafts in the bovine milk fat globule membrane revealed by the lateral segregation of phospholipids and heterogeneous distribution of glycoproteins.
Lopez C et al
Food Chemistry, 120.1, 22-33 (2010)
Wenwen Yu et al.
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The conversion of barley starch to sugars is a complex enzymic process. Most previous work concerned the biotechnical aspect of in situ barley enzymes. However, the interactions among the macromolecular substrates and their effects on enzymic catalysis has been little
Simon C Drew et al.
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The amyloid-β (Aβ) peptide is the primary component of the extracellular senile plaques characteristic of Alzheimer's disease (AD). The metals hypothesis implicates redox-active copper ions in the pathogenesis of AD and the Cu(2+) coordination of various Aβ peptides has been
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3 Reviews This action will navigate to reviews.

Questions

1–10 of 25 Questions  

  1. How much should one tablet weigh for it to yield 200ml 1xPBS? Asking just to make sure we won't use chipped tablets.

    1 answer

    1. Each tablet of this product should weigh between 1814.5 and 2005.5 milligrams.

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. If I want to make the same buffer molarity as the product P4474-1L, how many tablets should I dissolve in 1L of DI water?

    1 answer

    1. P4417 and P4474 have different components and initial concentrations. One P4417 tablet dissolved in 200 mL of deionized water yields a 0.01 M phosphate buffer, 0.0027 M potassium chloride, and 0.137 M sodium chloride at pH 7.4, 25 °C. P4474 contains 1.058 mM potassium phosphate monobasic, 154 mM sodium chloride, and 2.97 mM sodium phosphate dibasic dihydrate. Due to these differences, it is not feasible to directly match the molarity of P4474 by dissolving P4417 tablets.

      Helpful?

  5. Going by instructions, 1 tab in 200 mL water gives a 1x PBS sol'n. The recipe I use for 1x PBS has the same salt concs. Sorry if this is a silly question, but can I make up a 3x PBS solution simply by dissolving 3 tablets in 200 mL of water?

    1 answer

    1. These tablets have not been tested for solubility or dissolution time at a 3X concentration. The end user would have to determine solubility experimentally. Note that 10X prepared solutions are available and may easily be diluted to the desired concentration. Please see the link below to review several of these product options:
      https://www.sigmaaldrich.com/search/pbs-10x?focus=products&page=1&perpage=30&sort=relevance&term=PBS%2010X&type=product

      Helpful?

  6. Hi! If with this product I want to do a 10x solution, do i dilute it in 2000 mL or first in 200mL and then do the dilution? Thanks!

    1 answer

    1. Dissolve 1 tablet in 200 ml of water to obtain: 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4 At 25 °C. These instructions are for a 1 X PBS preparation and this product is only intended for this specific concentration. If a 10X concentrate is needed, there are options in the catalog, such as the following products : 79383, P7059, 6506-OP, and 6505-OP.

      Helpful?

  7. Is it possible to obtain the salt components for product 6501-200TAB? Additionally, can you provide the composition details for other PBS tablets or powder products?

    1 answer

    1. The compositions are as follows:

      1. 6501-200TAB: This PBS contains 137mM NaCl, 2.7 mM KCl, and 10mM Na phosphate.

      2. P3813 - powder - Packed in Pouch or PAK: Each pouch contains 8 g Sodium Chloride, 0.2 g Potassium Phosphate, monobasic, 1.15 g Sodium Phosphate, dibasic, and 0.2 g Potassium Chloride, totaling 9.55 g per packet. This is a standard PBS formulation.

      3. P4417- Tablet: When dissolved in 200 mL of deionized water, one tablet yields 0.01 M phosphate buffer, 0.0027 M potassium chloride, and 0.137 M sodium chloride, at pH 7.4 and 25 °C. This product contains the same formulation as P3813 on a per liter basis (excluding tableting fillers): 8 g/L Sodium Chloride, 0.2 g/L Potassium Phosphate, monobasic, 1.15 g/L Sodium Phosphate, dibasic, and 0.2 g/L Potassium Chloride. This mixture should be diluted to one liter of water, resulting in a pH of 7.4, which can be adjusted using phosphoric acid or sodium hydroxide. The concentrations are 10 mM phosphate buffer, 2.7 mM potassium chloride, and 137 mM. These PBS tablets do not contain BSA.

      Helpful?

  8. What is the osmolality of P4417 made to 1X PBS at pH 7.4?

    1 answer

    1. This PBS once diluted in 200 mL distilled water will account for:
      2.7 mM potassium chloride = 5.4 mosmoles / L
      137 mM sodium chloride = 274 mosmoles / L

      The exact osmolality is not determined.

      Helpful?

  9. Is this PBS calcium-free?

    1 answer

    1. The formulation of this product is considered proprietary. However, calcium is not included in the composition.

      Helpful?

  10. What is the purity and CAS

    1 answer

    1. This product is a mixture of components. CAS numbers are not assigned to multi-component products. Likewise, as there are multiple components, purity is not reported. Upon reconstitution according to instructions, the solution will contain 0.01 M phosphate buffer, 0.0027 M potassium chloride, and 0.137 M sodium chloride.

      Helpful?

1–10 of 25 Questions  

Reviews

1 of 3 reviewers received a sample product or took part in a promotion
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  1. Barcelona
    • Review 1
    • Vote 1
    5 out of 5 stars.

    Facilita la vida

    Durante mi tesis tenia que utilizar grandes cantidades y el poder hacerlo rapidamente con estas pastillas, te salva la vida

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  2. SETU, Kilkenny road , Carlow Ireland
    • Reviews 3
    • Votes 2
    5 out of 5 stars.

    good product

    arrived in time, very good product. I am happy with it

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  3. 1 Ratings-Only Review

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