This product is tested at pH 4.0 at 25C. The optimum pH and temperature have not been determined. However, the publication available at the link below may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5153499/#:~:text=The%20pectinase%20has%20an%20estimated,temperature%20of%2061%C2%B0C.
P4716
果胶酶 来源于黑曲霉
BioReagent, suitable for plant cell culture, aqueous glycerol solution, ≥5 units/mg protein (Lowry)
别名:
多聚半乳糖醛酸酶 溶液 来源于黑曲霉, 聚-（1,4-& # 945;-D·-半乳糖醛酸）聚糖水解酶
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国内现货，预计发货时间2025年4月07日详情
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5000 UNITS
$102.00
10000 UNITS
$187.00
25000 UNITS
$310.00
100000 UNITS
$927.00
About This Item
$102.00
国内现货，预计发货时间2025年4月07日详情
新价格，新优惠！
推荐产品
生物来源
Aspergillus niger
产品线
BioReagent
表单
aqueous glycerol solution
比活
≥5 units/mg protein (Lowry)
环保替代产品特性
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
技术
cell culture | plant: suitable
环保替代产品分类
, Enabling
储存温度
2-8°C
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相关类别
一般描述
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。该产品针对纤维素乙醇研究进行了优化，可提高能效并防止浪费。有关详细信息，请参阅《biofiles》中的文章和《替代能源研究用酶》
应用
用于植物原生质体制备，在细胞器分离前消化细胞壁。
生化/生理作用
从黑曲霉中选定菌株生成的果胶酶制剂：主要含有果胶转移酶、多聚半乳糖醛酸酶和果胶酯酶以及少量的半纤维素酶和纤维素酶。果胶酶水解果胶，果胶是细胞壁的一个组成部分。它们可能攻击甲基酯化果胶或去酯化果胶。它是果胶酶活性的来源，还含有纤维素酶和半纤维素酶活性。果胶酶催化果胶和其他半乳糖醛酸中的a-（1-4）-Dgalactosiduronic键的随机水解。
单位定义
在25°C条件下，当pH为4.0时，一个单位每分钟将释放从多聚半乳糖醛酸中得到的1.0 μ摩尔的半乳糖醛酸。
外形
40%甘油溶液
其他说明
有关复合碳水化合物分析 酶的更多信息，请访问 www.sigma-aldrich.com/enzymeexplorer
其他客户在看
Karyotypes of some species of Castanopsis, Lithocarpus and Quercus (Fagaceae) from Khun Mae Kuang Forest in Chiang Mai province, northern Thailand
Chokchaichamnankit P, et al.
Thai Forest Bulletin , 38-44 (2007)
Tony Heitkam et al.
The Plant journal : for cell and molecular biology, 103(1), 32-52 (2020-01-26)
If two related plant species hybridize, their genomes may be combined and duplicated within a single nucleus, thereby forming an allotetraploid. How the emerging plant balances two co-evolved genomes is still a matter of ongoing research. Here, we focus on
Tolerance mechanisms in mercury-exposed Chromolaena odorata (lf) RM King et H. Robinson, a potential phytoremediator
Alcantara HJP, et al.
Journal of Degraded and Mining Lands Management, 1(1), 09-20 (2013)
Trude Schwarzacher
Methods in molecular biology (Clifton, N.J.), 1370, 87-103 (2015-12-15)
Good preparations are essential for informative analysis of both somatic and meiotic chromosomes, cytogenetics, and cell divisions. Fluorescent chromosome staining allows even small chromosomes to be visualized and counted, showing their morphology. Aneuploidies and polyploidies can be established for species
Paulina Tomaszewska et al.
Annals of botany (2021-12-08)
Diploid and polyploid Urochloa (including Brachiaria, Panicum and Megathyrsus species) C4 tropical forage grasses originating from Africa are important for food security and the environment, often being planted in marginal lands worldwide. We aimed to characterize the nature of their
实验方案
To measure pectinase activity, a titrimetric stop reaction assay is used. One unit of pectinase will liberate 1 μmol of galacturonic acid from poly-galacturonic acid per hour at pH 4.0 at 25 °C.
在测定果胶酶活性时，使用了一种滴定终止反应法进行测定。在25 °C、pH 4.0条件下，一个单位的果胶酶每小时可从多聚半乳糖醛酸释放1 μmol 的半乳糖醛酸。
相关内容
A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.
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Hello! Is 25 degrees Celsius and pH 4.0 the optimal temperature and pH for the highest activity of this enzyme? or do I still need to check? Thanks!
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