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Merck

P3026

Sigma-Aldrich

溶果胶酶 来源于日本曲霉

greener alternative

lyophilized powder, ≥0.3 units/mg solid

别名:

果胶水解酶

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100 MG
$416.00
250 MG
$545.00
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$1,470.00

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100 MG
$416.00
250 MG
$545.00
1 G
$1,470.00

About This Item

MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

$416.00


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生物来源

Aspergillus sp. (A. japonicus)

质量水平

表单

lyophilized powder

比活

≥0.3 units/mg solid

分子量

50 kDa

环保替代产品特性

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

环保替代产品分类

储存温度

2-8°C

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一般描述

我们致力于为您提供更环保的替代产品,以符合“绿色化学的12项原则”的一项或多项原则要求。该产品针对纤维素乙醇研究进行了优化,可提高能效并防止浪费。有关详细信息,请参阅《biofiles》中的文章《替代能源研究用酶》

果胶酶或称果胶裂解酶在40-50°C的温度范围内稳定,它是一种50 kDa的酶,等电点pH为3.8。[1]

应用

可用于植物原生质体的制备,以在细胞器分离之前消化细胞壁。
可用于植物原生质体的制备,以在细胞器分离之前消化细胞壁。它可用于复杂碳水化合物的分析。产品P3026以含乳糖冻干粉形式提供。 产品P3026已用于在减数分裂分析之前消化景天树根尖[2]
来自日本曲霉的果胶水解酶已被用于:
  • 裂解小球藻细胞生成原生质体[3]
  • 作为拟南芥减数分裂染色体分离的酶消化缓冲液的成分[4]
  • 诱导 拟南芥根冠细胞结构的重组 [5]

生化/生理作用

果胶酶可催化 a-(1- 4)-D-半乳糖醛酸甲酯的消除性裂解,得到在非还原端具有4-脱氧-6-O-甲基-a-D-半乳糖-4-烯脲基的寡糖。 它包含两种果胶酶内多聚半乳糖醛酸酶和内切果胶裂解酶,以及浸渍刺激因子。
果胶酶用于从植物细胞中分离原生质体。[6]它还介导乙烯激素的合成。[6]

其他说明

据报道,其含有两种类型的果胶酶、内切多聚半乳糖醛酸酶(EC3.2.1.15)、内切蛋白裂解酶(EC4.2.2.10)以及浸渍刺激因子。

单位定义

在25 °C条件下,当pH为5.5时,一个单位每分钟将释放从多聚半乳糖醛酸中得到的1.0 μ摩尔的半乳糖醛酸。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Protocol for the preparation of Arabidopsis meiotic chromosome spreads and fluorescent in situ hybridization
Bolanos-Villegas P, et al.
The Plant Journal, 4, e1102-e1102 (2014)
Pectic enzymes in pectolyase: separation, characterization, and induction of ethylene in fruits
Baldwin EA and Pressey R
Plant Physiology, 90(1), 191-196 (1989)
Isolation and properties of pectinases from the fungus Aspergillus japonicus
Semenova MV, et al.
Biochemistry (Moscow), 68(5), 559-569 (2003)
Identification of sporopollenin as the outer layer of cell wall in microalga Chlorella protothecoides
He X, et al.
Frontiers in Microbiology, 7, 1047-1047 (2016)
P J De Lange et al.
Annals of botany, 101(6), 881-899 (2007-12-07)
Crassula hunua and C. ruamahanga have been taxonomically controversial. Here their distinctiveness is assessed so that their taxonomic and conservation status can be clarified. Populations of these two species were analysed using morphological, chromosomal and DNA sequence data. It proved

相关内容

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

Questions

1–3 of 3 Questions  
  1. How to dissolve the lyophilized powder of P3026 coded pectolyase powder? and how to prepare 0.8% solution of it?

    1 answer
    1. This product can be dissolved in cold deionized water up to 1000 units per mL. The units per mg are batch-dependent and can be found in the Certificate of Analysis.

      Helpful?

  2. Hello! I want to ask how much cellulase does the pectolyase (P3026) contains? It does not mention any contamination of other enzymes in the product description, but I want to know if there is any potential contamination involved.

    1 answer
    1. This product is not tested for the presence of contaminating enzymes. The primary components are endo-polygalacturonase and endo-pectin lyase. A brief search of the literature shows that in protoplast isolation, cellulase is often added to the digest, in addition to pectolyase.

      Helpful?

  3. Hello, I wanted to ask if this enzyme is equivalent to pectolyase Y-23, as the activity values are expressed differently.

    1 answer
    1. Yes, this product is equivalent to pectolyase Y-23.

      Helpful?

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