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Merck

P0029

Sigma-Aldrich

蛋白酶 来源于芽孢杆菌

别名:

复合蛋白酶®

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50 G
$246.00
250 G
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About This Item

EC 号:
3.4.21.14
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54

$246.00


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生物来源

Bacillus sp.

表单

granular

enzyme activity

>1.5 AU-N/g

储存温度

2-8°C

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相关类别

一般描述

来自芽孢杆菌的蛋白酶在制药、皮革、食品和废物处理行业中具有广泛的用途。它广泛用于生产蛋白质水解物。[1]芽孢杆菌蛋白酶的稳定性和活性进行分析表明,该酶在5.0-11.0的任何pH值下均具有活性,在8.0时具有100%的活性。经确定,蛋白稳定性的最佳pH为7.0,稳定性的最佳温度为60°C,并可保持95%的活性。[2]

应用

来自芽孢杆菌的蛋白酶已被用于:
  • 评估蛋白酶抑制剂的活性[3]
  • 使用响应面法在间歇发酵中获得高产量[4]
  • 在一项研究中研究一种生物传感器,以检测一种来自于地衣芽孢杆菌的蛋白酶生物标志物[5]

分析说明

最小活性 1.5 AU-N/G 固体

法律信息

Protamex is a registered trademark of Novozymes Corp.

象形图

Health hazardExclamation mark

警示用语:

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Hypocholesterolemic effect of compounded freshwater clam protein hydrolysate and Gracilaria
Lin, Y-H, et al
Food Chemistry, 123(2), 395-399 (2010)
Huiwang Wu et al.
The Analyst, 137(20), 4829-4833 (2012-08-30)
A simple, selective and sensitive electrochemical biosensor has been developed to detect protease biomarker from Bacillus licheniformis, a recognized model of the biochemical warfare agent Bacillus anthracis. In this assay, the biosensor is constructed using a d-amine acid containing substrate
Anum Khan et al.
Molecular biology reports, 46(4), 4565-4580 (2019-06-28)
Polysaccharides including resistant starch are categorized as dietary fiber and are used as an important prebiotic. Similar to soluble fibers, resistant starch also has a number of physiological effects that have been shown to be beneficial for health. Starch hydrolyzing
Trypsin Inhibitor from Edible Mushroom Pleurotus floridanus Active against Proteases of Microbial Origin
<BIG>Ali PPM, et al.</BIG>
Applied Biochemistry and Biotechnology, 167-178 (2014)
Characterization of protein hydrolysates and lipids obtained from black scabbardfish (Aphanopus carbo) by-products and antioxidative activity of the hydrolysates produced.
Batista, I., et al
Process. Biochem., 45(1), 18-24 (2010)

Questions

1–2 of 2 Questions  
  1. Podrían indicarme las temperaturas y pH óptimos de trabajo de esta proteasa

    1 answer
    1. As mentioned in the 'DESCRIPTION' section, the optimal pH for protein stability was determined to be at 7.0 while the optimal temperature for stability was at 60°C with 95% activity maintained.

      Helpful?

  2. What is the composition (or protein content) of this protease?

    1 answer
    1. This is a product of Novozymes. The composition is considered proprietary, however, it is a blend of aggressive endo-proteases.

      See the link below to a publication that may be helpful:
      https://www.sciencedirect.com/science/article/pii/S2772502222000683

      Helpful?

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