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Merck

NUC101

Sigma-Aldrich

细胞核分离试剂盒:Nuclei EZ Prep

sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)

别名:

Rapid mammalian nuclei isolation kit

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.32

用途

sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)

品質等級

儲存期限

1 yr at 2‑8 °C

包裝

pkg of 1 kit

應用

cell analysis

異物活動

nuclease and protease, free

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

Nuclei EZ Prep Kit用于从哺乳动物细胞中快速分离胞核。该试剂盒可从通用细胞类型获得高得率细胞核。

應用

适于作为细胞核成分的来源,为体外凋亡检测和功能研究提供细胞核成分。

生化/生理作用

配套实验方案使您可从常用的哺乳动物细胞获得高得率的细胞核,包括贴壁 (例如,HEK293和COS7)和非贴壁(例如,Jurkat和HFN7.1)细胞系和外周血单核细胞(PBMC)。该制备适用于许多细胞生物学应用,例如:为染色质、基因组DNA、组蛋白和核RNA/RNP提供细胞核来源,为体外凋亡检测、功能性研究(如细胞转录状态检查)制备细胞核。

其他說明

所有操作步骤应在冰块上或2-8°C条件下进行。

相關產品

产品编号
说明
价格

儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Christina N Vallianatos et al.
Communications biology, 3(1), 278-278 (2020-06-03)
Histone H3 lysine 4 methylation (H3K4me) is extensively regulated by numerous writer and eraser enzymes in mammals. Nine H3K4me enzymes are associated with neurodevelopmental disorders to date, indicating their important roles in the brain. However, interplay among H3K4me enzymes during
Analysis of nuclear RNA, Chapter 14.
Robert E. Farrell, Jr., ed.
RNA Methodologies: A Laboratory Guide for Isolation and Characterization, 235-263 (1993)
Buqing Ye et al.
Nature communications, 8(1), 1518-1518 (2017-11-16)
Lymphoid lineage commitment is an important process in haematopoiesis, which forms the immune system to protect the host from pathogen invasion. However, how multipotent progenitors (MPP) switch into common lymphoid progenitors (CLP) or common myeloid progenitors (CMP) during this process
Yu-Yin Shih et al.
PloS one, 6(10), e26236-e26236 (2011-10-25)
Retinoic acid (RA) has been approved for the differentiation therapy of neuroblastoma (NB). Previous work revealed a correlation between glucose-regulated protein 75 (GRP75) and the RA-elicited neuronal differentiation of NB cells. The present study further demonstrated that GRP75 translocates into
Baochen Shi et al.
BMC genomics, 10, 92-92 (2009-02-27)
A major goal of post-genomics research is the integrated analysis of genes, regulatory elements and the chromatin architecture on a genome-wide scale. Mapping DNase I hypersensitive sites within the nuclear chromatin is a powerful and well-established method of identifying regulatory

商品

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

亚细胞组分离心分离是一种常用技术,广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同,因此通过离心,可从温和均质的样品中轻松分离和纯化细胞器组分。

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