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Merck
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文件

I8896

Sigma-Aldrich

IGEPAL® CA-630

for molecular biology

别名:

辛基苯氧基聚（乙烯氧基）乙醇，支链, 聚氧乙烯辛烷基苯酚醚，分支, 辛基苯基-聚乙烯二醇

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About This Item

线性分子式:
(C2H4O)nC14H22O
CAS号:
9002-93-1
分類程式碼代碼:
12352104
NACRES:
NA.31

等級

for molecular biology

描述

non-ionic

形狀

liquid

技術

IR spectroscopy: suitable

CMC

0.08 mM (20-25°C)

HLB

13

異物活動

DNase and RNase, none detected

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

InChI 密鑰

JYCQQPHGFMYQCF-UHFFFAOYSA-N

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相关类别

一般說明

IGEPAL® CA-630是一种非离子型、非变性去污剂，适用于膜蛋白复合物的溶解、分离和纯化。它与Nonidet P-40在化学上无法区分。

應用

IGEPAL®CA-630已用于
  • 在小鼠肌肉匀浆的western blotting中作为匀浆缓冲液中的中性洗涤剂
  • 研究E8.5-E10.5胚胎中lacZ表达的X-gal染色
  • 制备可用于RT-qPCR的细胞裂解物的细胞裂解（CL）缓冲液

其他說明

非离子型、非变性洗涤剂

品質

在化学性质方面，不能与已经退市的 Nonidet P-40 区分开。

注意

在贮存过程中可能产生混浊或沉淀物。 可在加热至时40°C变为澄清液体。

法律資訊

IGEPAL is a registered trademark of Solvay

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

483.8 °F - closed cup

閃點（°C）

251 °C - closed cup

個人防護裝備

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

分析证书(COA)

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Joseph A Roche et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 59(11), 964-975 (2011-11-02)
Mutations in the DYSF gene that severely reduce the levels of the protein dysferlin are implicated in muscle-wasting syndromes known as dysferlinopathies. Although studies of its function in skeletal muscle have focused on its potential role in repairing the plasma
Bojana Lucic et al.
Nature communications, 10(1), 4059-4059 (2019-09-08)
HIV-1 recurrently targets active genes and integrates in the proximity of the nuclear pore compartment in CD4+ T cells. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained
Amina Abbadi et al.
The Journal of biological chemistry, 291(3), 1448-1455 (2015-11-26)
Many cells, including murine airway epithelial cells, respond to a variety of inflammatory stimuli by synthesizing leukocyte-adhesive hyaluronan (HA) cables that remain attached to their cell surfaces. This study shows that air-liquid interface cultures of murine airway epithelial cells (AECs)
Simon Lalonde et al.
Genome biology, 20(1), 133-133 (2019-07-10)
Genome-wide association studies (GWAS) have identified hundreds of loci associated with coronary artery disease (CAD) and blood pressure (BP) or hypertension. Many of these loci are not linked to traditional risk factors, nor do they include obvious candidate genes, complicating
Kenneth Shatzkes et al.
Scientific reports, 4, 4659-4659 (2014-04-12)
Sample nucleic acid purification can often be rate-limiting for conventional quantitative PCR (qPCR) workflows. We recently developed high-throughput virus microneutralization assays using an endpoint assessment approach based on reverse transcription qPCR (RT-qPCR). The need for cumbersome RNA purification is circumvented
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Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

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