The assay buffer has a pH of 7.4. It is recommended to maintain the sample pH closer to 7.4, as highly alkaline pH may interfere with the reaction.
推荐产品
用途
sufficient for 100 fluorometric tests
检测方法
fluorometric
一般描述
适用性
原理
警示用语:
Danger
危险分类
Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1
储存分类代码
3 - Flammable liquids
闪点(°F)
49.5 °F
闪点(°C)
9.7 °C
历史批次信息供参考:
-
Could you provide the pH of the Assay buffer in the MAK363 Lipoxygenase Activity Assay Kit? Also, does the pH of the final solution interfere with the assay? If protein samples, extracted with high pH (10.8 buffer), are too diluted and need to add more samples, would the high pH of the samples inhibit the assay reaction?
1 answer-
Helpful?
-
-
What is the concentration of the enzyme?
1 answer-
The concentration of the enzyme standard for this kit is not reported. While it does confirm that the kit is functioning, it is not to be compared to samples or intended as a control. In order to quantify the LOX activity of the sample, please use the formula in step 6 on page 4 of the data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/247/994/mak363bul-ms.pdfHelpful?
-
Active Filters
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门