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MAK307

Sigma-Aldrich

孔雀绿磷酸盐检测试剂盒

sufficient for 2500 colorimetric tests

别名:

磷酸盐测定试剂盒

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1 KIT
$482.00

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1 KIT
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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

$482.00

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获取大包装报价

用途

sufficient for 2,500 colorimetric tests

檢測方法

colorimetric

相關疾病

cardiovascular diseases; gastrointestinal diseases

儲存溫度

2-8°C

相关类别

一般說明

磷酸盐是人体内含量最丰富的一种矿物质，含量约占总体重的1％。甲状旁腺激素（PTH）、钙三醇和调磷因子等多种调节剂共同维持磷酸盐稳态。维持磷酸盐稳态对于各种生物过程至关重要，生物过程包括能量代谢、细胞信号传导、蛋白质合成调节、骨骼发育和骨骼完整。†生长板中成熟软骨细胞的凋亡过程需要足够水平的磷酸盐。磷酸盐缺乏与骨骼病理和临床疾病有关。†

應用

孔雀石绿磷酸盐检测试剂盒已被用于测定以下应用中游离正磷酸盐的释放：
  • 磷酸酶检测：从肽、蛋白质或小分子底物中释放磷酸盐。[1][2]
  • 脂肪酶检测：从磷脂中释放磷酸盐
  • 核苷三磷酸酶检测：从核苷三磷酸（ATP、GTP、TTP、CTP等）中释放磷酸盐。
  • 磷脂、蛋白质和DNA等中磷酸盐的定量。
  • 药物发现：磷酸酶抑制剂的高通量筛选。
孔雀石绿磷酸盐测定试剂盒用于磷酸盐定量[1] 和稳态动力学研究。[3]

特點和優勢

能与高通量处理系统兼容。

適合性

适用于各种生物和环境样品中的磷酸盐测定。

原則

孔雀石绿是一种属于三苯甲烷基团的碱性染料。它在生物学中可用于显微镜样品的染色，例如植物组织、酵母和细菌。孔雀石绿法是检测磷酸盐释放的灵敏方法。孔雀石绿磷酸盐测定试剂盒基于对孔雀石绿、钼酸盐和游离正磷酸盐之间形成的绿色复合物的定量。可以在分光光度计（600-660nm）或读板器上对反应中快速颜色形成进行方便地测定。该非放射性比色测定试剂盒已经过优化，可提供卓越的灵敏度和更长的保质期。该测定简单且快速，只需要单一的添加步骤，以对磷酸盐进行测定。可以在管、比色皿或多孔板中进行测定。可以在96孔和384孔板中方便地进行测定，以用于酶抑制剂的高通量筛选。

象形圖

FlameCorrosion
GHS02,GHS05

訊號詞

Warning

危險分類

Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2

儲存類別代碼

3 - Flammable liquids

閃點（°F）

82.4 °F

閃點（°C）

28 °C

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
307CE08A20
307CE08A13
307CE03A11
307CD12A06
307CD08A28

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Questions

1–9 of 9 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. Is this kit able to detect pyrophosphate too?

    1 answer

    1. This kit is intended for the detection of free orthophosphate and has not been tested for pyrophosphate detection. There are other products available that are specifically for use in pyrophosphate detection. Please see the links below to review each item:
      MAK168, Pyrophosphate Assay Kit
      https://www.sigmaaldrich.com/product/sigma/mak168
      MAK169. High Sensitivity Pyrophosphate Assay Kit
      https://www.sigmaaldrich.com/product/sigma/mak169

      Helpful?

  3. What are the reasons behind the suggestion to use lower concentrations than 0.25mM? Are there potential unwanted biochemical reactions between GTP and malachite green/molybdate if GTP concentration exceeds 0.25mM?

    1 answer

    1. The kit operates by releasing free orthophosphate from ATP or GTP, followed by the creation of a green complex involving the orthophosphate, molybdate, and Malachite green. It has been observed that samples containing ATP and GTP at concentrations exceeding 0.25mM result in the measurement of free phosphate levels falling outside the detection range of the kit (0.02 µM and 40 µM phosphate), and hence should be avoided.

      Helpful?

  4. Does the product MAK307 contain magnesium?

    1 answer

    1. Magnesium is not included as a component in any of the reagents provided with the kit for product MAK307. If magnesium serves as an essential cofactor for the enzyme, it is recommended to supplement it in the buffer before conducting the phosphate determination assay.

      Helpful?

  5. Why is it necessary to dilute the ATP concentration to <0.25 mM before the assay? Does ATP interfere with the assay?

    1 answer

    1. BioAssay Support recommends reducing the ATP concentration before the assay to minimize background interference resulting from non-enzymatic ATP hydrolysis and to decrease the likelihood of reagent precipitation with unhydrolyzed ATP.

      Helpful?

  6. our kit expired, when I used to measure extracted polyphosphate degraded by the enzyme, the results were so different compared to the precious ones (when the kit was not expired), this means the result was not reliable after it expired?

    1 answer

    1. Correct, products used beyond the expiration date will likely offer poor results.

      Helpful?

  7. Does Malachite Green interact with glycerol?

    1 answer

    1. The Malachite Green Phosphate Assay Kit is based on the quantification of the green complex formed between Malachite Green, molybdate, and free orthophosphate. No specific compatibility study has been done with this kit and glycerol. This would need to be tested empirically, in a sample with a known phosphate concentration, with and without glycerol at the desired concentration.

      Helpful?

  8. Can we test the same MG in shrimp matrix ?

    1 answer

    1. This product is suitable for a variety of sample types. There is no reason to suspect a problem with shrimp matrix. Attention should be given to sample preparation, handling, and dilution. See the link below to review the product datasheet which includes the protocol and troubleshooting tips:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/171/212/mak307bul.pdf

      Helpful?

  9. How is shipping temperature determined? And how is it related to the product storage temperature?

    0 answers

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