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MAK168

Sigma-Aldrich

焦磷酸测定试剂盒

sufficient for 200 fluorometric tests (Blue fluorescence)

别名:

高灵敏度焦磷酸检测试剂盒

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1 KIT
$476.00

$476.00

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1 KIT
$476.00

About This Item

EC 号:
200-664-3
UNSPSC代码:
12161503
NACRES:
NA.84

$476.00

请联系客服了解存货情况
获取大包装报价

用途

sufficient for 200 fluorometric tests (Blue fluorescence)

应用

cosmetics
food and beverages

检测方法

fluorometric

储存温度

−20°C

相关类别

一般描述

焦磷酸 (PPi) 通过多次生化反应而生出，如 ATP 水解、[1]DNA 和 RNA 聚合、环 AMP 形成以及脂肪酸-辅酶 a 酯的形成。其被无机焦磷酸酶水解。PPi 存在于血液和组织的细胞外基质中。[1]

适用性

焦磷酸检测试剂盒为用户提供了简单直接的微孔板分析方法，可测定多种样本中的焦磷酸水平。

原理

通过使用独特的荧光焦磷酸传感器测定样品的焦磷酸浓度，其中焦磷酸的存在导致产生与焦磷酸成比例的荧光产物 (λex = 316/λem = 456 nm)。该检测方法比传统的基于酶的方法更简单、更耐用，是筛选酶活性或酶抑制剂的理想方法。

象形图

Corrosion
GHS05

警示用语：

Danger

危险声明

H318

预防措施声明

P280 - P305 + P351 + P338

危险分类

Eye Dam. 1

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

188.6 °F

闪点(°C)

87 °C

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
3510613
3510613
3041242
3320133
2920633

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Peiyi Zheng et al.
Journal of molecular biology, 431(4), 764-776 (2019-01-18)
Phosphopantothenoylcysteine (PPC) synthetase (PPCS) catalyzes nucleoside triphosphate-dependent condensation reaction between 4'-phosphopantothenate (PPA) and l-cysteine to form PPC in CoA biosynthesis. The catalytic mechanism of PPCS has not been resolved yet. Coenzyme A biosynthesis protein 2 (Cab2) possesses activity of PPCS
Pyrophosphate hydrolysis is an intrinsic and critical step of the DNA synthesis reaction.
Kottur J and Deepak T N
Nucleic Acids Research (2018)
Tyler M Weaver et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(41), 25494-25504 (2020-10-02)
During DNA replication, replicative DNA polymerases may encounter DNA lesions, which can stall replication forks. One way to prevent replication fork stalling is through the recruitment of specialized translesion synthesis (TLS) polymerases that have evolved to incorporate nucleotides opposite DNA
Jithesh Kottur et al.
Nucleic acids research, 46(12), 5875-5885 (2018-06-01)
DNA synthesis by DNA polymerases (dPols) is central to duplication and maintenance of the genome in all living organisms. dPols catalyze the formation of a phosphodiester bond between the incoming deoxynucleoside triphosphate and the terminal primer nucleotide with the release
Priyanka Kushwaha et al.
Journal of cellular physiology, 235(10), 6673-6683 (2020-01-28)
The activation of the Wnt/β-catenin signaling pathway is critical for skeletal development but surprisingly little is known about the requirements for the specific frizzled (Fzd) receptors that recognize Wnt ligands. To define the contributions of individual Fzd proteins to osteoblast
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Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. Any challenges encountered with decreases in fluorescence due to buffer components in the assays, such as manganese (1 mM), DTT (5 mM), and the combination of manganese with a reducing agent (both BME and DTT)? It seems that the signal is significantly reduced, if not entirely abolished, when these components are included in the buffer, even with substantial amounts of the PPi standard.

    1 answer

    1. Considering the significant quenching of fluorescence by manganese (Mn), it is not suitable for the assay. Instead, the use of MgCl2 (up to 1 mM) is suggested as it offers less interference. Additionally, DTT can be used up to 1 mM.

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