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Merck

MAK499

Sigma-Aldrich

Glutamate Dehydrogenase (GDH) Assay Kit

sufficient for 100 colorimetric tests

别名:

Assay Kit for Glutamate Dehydrogenase Activity

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.84

品質等級

應用

agriculture

檢測方法

colorimetric

相關疾病

cancer; gastrointestinal diseases

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

Glutamate dehydrogenase (GLDH) is an enzyme which catalyzes the interconversion of glutamate and α-ketoglutarate. Elevated blood serum GLDH levels indicate liver damage; thus, GLDH plays an important role in the study of liver disease, especially in combination with aminotransferases. Transgenic plants expressing microbial GLDHs are improved in tolerance to herbicide, water deficit, and pathogen infections.

特點和優勢

  • Linear detection range: 0.4 to 80 U/L for 30 minute reaction. Detection Limit of 0.1 U/L for 120 minute reaction.
  • Compatible with high-throughput handling systems.

適合性

The kit is suitable for glutamate dehydrogenase activity determination in biological samples (e.g. plasma, serum, urine, tissue, and culture media.)

原則

The non-radioactive, colorimetric Glutamate Dehydrogenase (GDH) Assay Kit is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

訊號詞

Danger

危險分類

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

3 - Flammable liquids

水污染物質分類(WGK)

WGK 2


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