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Merck
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主要文件

MAK190

Sigma-Aldrich

ATP 检测试剂盒

Sufficient for 100 Colorimetric or Fluorometric tests

别名:

三磷酸腺苷检测试剂盒

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About This Item

EC 号:
213-579-1
UNSPSC代码:
12161503
NACRES:
NA.84

检测方法

colorimetric
fluorometric

相关疾病

cancer; neurological disorders

储存温度

−20°C

一般描述

三磷酸腺苷(ATP)是一种仅在线粒体内形成的三磷酸核苷。这是一种高能分子，被称为所有生命系统的能量货币。包含在ATP的磷酸键中的化学能量驱动着大多数的细胞过程。[1][2]诸如Leber遗传性视神经病变、[2]Leigh综合征、神经病、共济失调以及色素性视网膜炎[3]等遗传疾病都会影响线粒体内的ATP生成。

应用

ATP检测试剂盒已用于通过对样本中ATP的荧光检测而对ATP浓度进行量化。[4]

适用性

适于测定不同样品中的ATP，如动物组织和细胞培养样品。

原理

ATP比色/荧光检测试剂盒是一种用于测定各种样品中ATP的灵敏检测（比色检测为2-10 nmole/孔而荧光检测为20-1,000 pmole/孔）。ATP的浓度是通过磷酸化甘油而测定，可产生与存在的ATP量成比例的比色（570 nm）或荧光（lex = 535/lem = 587 nm）产物。

其他说明

荧光检测比比色检测具有10-100倍更高的灵敏度。

仅试剂盒组分

产品编号
说明
  • ATP Assay Buffer
  • ATP Probe, in DMSO
  • ATP Converter
  • Developer Mix
  • ATP Standard, 1.0 μmole

替代产品

产品编号
说明
价格

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

10 - Combustible liquids

闪点(°F)

188.6 °F

闪点(°C)

87 °C

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
8L09K03540
8E04K03540
8A07K03540
7H23K03540
7D05K03540

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访问文档库

Cost of surviving sepsis: A novel model of recovery from sepsis in Drosophila melanogaster.
Kaynar A M, et al.
Intensive care medicine experimental, 4(1), 4-4 (2016)
E Alirol et al.
Oncogene, 25(34), 4706-4716 (2006-08-08)
Mitochondria are key players in several cellular functions including growth, division, energy metabolism, and apoptosis. The mitochondrial network undergoes constant remodelling and these morphological changes are of direct relevance for the role of this organelle in cell physiology. Mitochondrial dysfunction
Brock A Humphries et al.
Breast cancer research : BCR, 22(1), 60-60 (2020-06-07)
Mitochondrial dynamics underlies malignant transformation, cancer progression, and response to treatment. Current research presents conflicting evidence for functions of mitochondrial fission and fusion in tumor progression. Here, we investigated how mitochondrial fission and fusion states regulate underlying processes of cancer
Melatonin Balance the Autophagy and Apoptosis by Regulating UCP2 in the LPS-Induced Cardiomyopathy.
Pan P, et al.
Molecules (Basel), 23(3), 675-675 (2018)
Sandra Aurora Niño et al.
ACS chemical neuroscience, 10(1), 323-336 (2018-08-25)
Worldwide, every year there is an increase in the number of people exposed to inorganic arsenic (iAs) via drinking water. Human populations present impaired cognitive function as a result of prenatal and childhood iAs exposure, while studies in animal models
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Questions

1–5 of 5 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the chemical composition of the reagents?

    1 answer

    1. The chemical composition of these reagents is proprietary.

      Helpful?

  4. Dear sir, We are interested in quantification of ATP levels "ATP Assay Kit" Cat.No:MAK190. Protocol recommends that cells should be deproteinized using 10kDa MWCO spin filter, but no protocol is mentioned. Can you Please send us protocol for the same.

    1 answer

    1. The Datasheet can be accessed in the DOCUMENTATION section under 'More Documents'. Please see the Datasheet below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/262/439/mak190bul.pdf

      Helpful?

  5. What is the chemical composition of the reagents?

    1 answer

    1. The chemical composition of these reagents are proprietary. Please see the link below to review the Product datasheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/262/439/mak190bul.pdf

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