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MAK151

Sigma-Aldrich

荧光硫醇测定试剂盒

sufficient for 200 fluorometric tests

别名:

硫醇荧光测定试剂盒

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1 KIT
$476.00

$476.00

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1 KIT
$476.00

About This Item

EC 号:
200-664-3
UNSPSC代码:
12161503
NACRES:
NA.84

$476.00

请联系客服了解存货情况
获取大包装报价

用途

sufficient for 200 fluorometric tests

检测方法

fluorometric

相关疾病

endocrinological disorders, diabetes; cancer

储存温度

−20°C

相关类别

一般描述

在游离半胱氨酸、谷胱甘肽（GSH）和蛋白质半胱氨酸残基中发现的硫醇基团可参与许多生物过程。当两个半胱氨酸残基的硫醇基团被氧化时，所产生的二硫键有助于蛋白质的三级或四级结构。

应用

荧光硫醇测定试剂盒已用于测定样品中以硫醇表示的脂质过氧化。[1]

适用性

该试剂盒适用于检测各种不同生物样品中的硫醇，包括血浆、尿液和细胞提取物。该试剂盒未针对测定蛋白质中的硫醇基团进行优化。

原理

荧光硫醇定量试剂盒可提供超灵敏的测定，可用于定量小分子中的硫醇含量。专有的硫醇感受分子在与含硫醇的化合物反应时可产生强荧光加合物(λex = 490/λem = 520 nm)。此外，硫醇加合物的吸收和发射光谱均与pH无关，使得该测定试剂盒非常稳定。该试剂盒可在100 μL测定体积(10 nM).内检测低至1 皮摩尔的半胱氨酸或GSH。可使用96或384多孔板形式方便地进行测定。

储存分类代码

10 - Combustible liquids

WGK

WGK 3

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
3510891
304162
3320703
2781663
2391623

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Questions

1–4 of 4 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. Can this kit be used to quantify cysteines from proteins? If so, do I need to reduce disulfide bonds first? Also, does maleimide interfere with the assay?

    1 answer

    1. This kit detects reduced thiols such as reduced Glutathione (GSH) and Cysteine. In addition, the product may also be used to detect SH groups in the protein, provided that a standard with known SH groups is used, such as BSA (0.5-1 GH/molecule). The possible interference of Meleimide has not been investigated.

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  4. Would MAK151 detect reduced thiols like GSH, Cysteine, and NAC, or does it detect total thiol? Additionally, does it detect total protein-SH or protein-S-S protein?

    1 answer

    1. MAK151 is capable of detecting reduced thiols like GSH, Cysteine, etc. It can also be used to detect SH groups in proteins, provided that known SH groups of the protein standard, such as BSA (0.5-1 GH/molecule), are available.

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