用途
sufficient for 100 colorimetric tests
检测方法
colorimetric
相关疾病
cancer
储存温度
−20°C
一般描述
Maleimide is an unsaturated imide[1] used in protein conjugation reactions.[2] The direct measurement of maleimide moieties following conjugation reactions by spectrophotometric analysis at 302 nm is insensitive due to the small extinction coefficient (620M-1 cm-1) and complicated by protein absorbance at this wavelength.
适用性
Suitable for quantifying maleimide groups
原理
The colorimetric maleimide quantiation kit provides a simple and direct procedure for measuring maleimide groups. The sample is first reacted with a known amount of excess thiol and then the remaining unreacted thiol is assayed using 4,4′-DTDP (4,4′-Dithiodipyridine). The amount of maleimide is calculated as the difference between the initial amount of thiol and the amount of unreacted thiol after the complete reaction of all maleimide groups.
警示用语:
Warning
危险分类
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Sens. 1 - STOT SE 3
靶器官
Respiratory system
储存分类代码
10 - Combustible liquids
闪点(°F)
188.6 °F - closed cup
闪点(°C)
87 °C - closed cup
Efficient site-specific labeling of proteins via cysteines.
Kim Y, et al.
Bioconjugate Chemistry, 19(3), 786-791 (2008)
Carbon Composites: Composites with Carbon Fibers, Nanofibers, and Nanotubes (2016)
Active Filters
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持