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MAK052

Sigma-Aldrich

ALT 活性测定

sufficient for 100 colorimetric or fluorometric tests

别名:

SGPT活性检测试剂盒, 丙氨酸转氨酶活性检测试剂盒, 血清谷丙转氨酶活性检测试剂盒

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1 KIT
$1,010.00

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1 KIT
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About This Item

UNSPSC代码:
12161503
NACRES:
NA.84

$1,010.00

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获取大包装报价

用途

sufficient for 100 colorimetric or fluorometric tests

检测方法

colorimetric
fluorometric

相关疾病

gastrointestinal diseases

运输

wet ice

储存温度

−20°C

基因信息

human ... GPT(2875)
mouse ... GPT(76282)
rat ... GPT(81670)

一般描述

丙氨酸氨基转移酶（ALT），也称为血清谷氨酸-丙酮酸转氨酶（SGPT），是一种吡哆醛-磷酸盐依赖型的酶，其可催化氨基从丙氨酸到α-酮戊二酸的可逆型转移，并生成丙酮酸和谷氨酸。ALT主要在肝脏和血清中被发现，但也存在于其他组织中。肝细胞损伤经常会导致血清ALT水平的升高而血清ALT水平可被用作肝损伤的一种标志物。

应用

ALT活性检测试剂盒已用于确定血清样品中丙氨酸氨基转移酶（ALT）的活性。[1][2][3]

特点和优势

兼容高通量处理系统。

适用性

适于测定组织、细胞和血清等生物学样品中的丙氨酸转氨酶（ALT）活性。

原理

ALT活性检测试剂盒可提供在多种生物学样本进行简单而直接的ALT活性测定过程。

ALT活性由一种偶联酶检测所确定，其可产生与生成的丙酮酸盐成比例的比色（570 nm）/荧光（λex = 535/λem = 587 nm）产物。一个单位的ALT定义为在 37 °C每分钟可生成1.0 μ摩尔丙酮酸所需的酶量。

相关产品

产品编号
说明
价格

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H317,H334,H412

危险分类

Aquatic Chronic 3 - Resp. Sens. 1 - Skin Sens. 1

储存分类代码

10 - Combustible liquids

WGK

WGK 3

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
8L18K07520
8L23K07520
8A24K07520
8K03K07520
8K11K07520

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Questions

1–8 of 8 Questions  

  1. Hi, Does this ALT kit depend on LDH for the ultimate readout? Our goal is to measure liver dysfunction in serum of mice. Would differences in serum LDH influence the results? Thanks Ricky

    1 answer

    1. This kit does not use lactate dehydrogenase (LDH) itself, but the measured results are proportional to the concentration of pyruvate generated. Because LDH (in the presence of NADH) can influence the concentration of pyruvate present, the presence of LDH in a sample may influence the assay results.

      LDH was not assessed in the development of this kit, but the kit has been used to test serum samples directly. It is recommended to perform several sample dilutions to determine the optimal sample concentration and ensure measured values fall within the linear range of the standard curve. Liver cell and liver-derived cell line lysates are more commonly measured, as serum levels of ALT are normally low. Regardless, considering the effect of LDH on pyruvate in the presence of NADH, a variable concentration of LDH and/or NADH may impact the ALT assay results.

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  4. Can I know the specific activity of this ALT mix sample? In unit of U/mg

    1 answer

    1. The ALT Enzyme Mix included in this kit is a lyophilized mixture of enzymes. The components include Pyruvate Oxidase, Cocarboxylase, and Sucrose. The concentration and activity of each enzyme are considered proprietary. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/731/802/mak052bul.pdf

      Helpful?

  5. Is the ALT activity assay kit MAK052 compatible with EDTA plasma? Does EDTA interfere with the activity of the assay?

    1 answer

    1. EDTA has the potential to deactivate the enzyme. ​Therefore, it is recommended not to use samples containing EDTA for performing the AST and ALT assay kits, including the MAK055 kit.

      Helpful?

  6. How does the presence of pyruvate in the Krebs buffer affect the final reaction of the kit, and why is its removal recommended? Additionally, what is the significance of the absence of enzymes in the kit for converting glucose and lactate to pyruvate in relation to the buffer?

    1 answer

    1. The kit's final reaction is dependent on the oxidation of pyruvate, so its presence in the buffer may interfere with the assay. If possible, it is recommended to remove pyruvate from the Krebs buffer. However, the kit does not contain enzymes that can convert glucose to pyruvate or lactate to pyruvate, so the presence of these should not pose an issue in the buffer.

      Helpful?

  7. Can I use the kit for both mouse and human serum?

    1 answer

    1. This kit is suitable for mouse and human serum. As mentioned on the Product Detail Page, this item is suitable for measuring alanine aminotransferase (ALT) activity in biological samples including tissue, cells, and serum.

      Helpful?

  8. Can it be used for measuring on supernatant from liver cells

    1 answer

    1. The kit is optimized for use in determination of alanine aminotransferase in biological samples (e.g. plasma, serum, cell lysate, tissue samples.) This kit has not been tested for use with cell culture supernatant. The presence of phenol red may interfere with results. In addition, the cultured cell line may not secrete sufficient ALT to be detected. It would be up to the end user to determine suitability.

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