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Merck

MAK009

Sigma-Aldrich

淀粉酶活性检测试剂盒

sufficient for 100 colorimetric tests

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84
价格与库存信息目前不能提供

用途

sufficient for 100 colorimetric tests

檢測方法

colorimetric

相關疾病

endocrinological disorders, diabetes; gastrointestinal diseases

儲存溫度

−20°C

基因資訊

human ... AMY1A(278)

一般說明

现在我们可提供全新的淀粉酶检测试剂盒MAK478!淀粉酶是一种糖苷水解酶,它能裂解多糖(如淀粉或糖原)中的葡聚糖键,从而释放多糖分子。α-淀粉酶存在于动物、植物和许多真菌中。[1]在哺乳动物中,α-淀粉酶是淀粉酶的主要形式,是膳食消化的主要酶之一。α-淀粉酶的活性和稳定性都依赖于钙离子。人体α-淀粉酶的水平在胰腺和唾液分泌物中最高。[2]血液和尿液淀粉酶水平的升高是由于胰腺疾病和唾液腺损伤引起的。唾液淀粉酶活性的增加也是交感神经系统活性增加的一个标志。[3][4]

應用

采用淀粉酶活性测定试剂盒已可用于测定淀粉酶的活性。[5][6][7]

適合性

适用于定量测定各种生物样品中的淀粉酶活性。

原則

淀粉酶活性测定试剂盒可定量测定多种生物样品中的淀粉酶活性。该试剂盒快速,方便并且灵敏。在该试剂盒中,淀粉酶活性是通过耦合酶法测定的,其产生一个比色(405 nm)的产品,与底物的数量成比例的乙烯-pNP-G7,由淀粉酶裂解得到。一个单位的淀粉酶,在25℃下,每分钟可裂解乙烯-pNP-G7而产生1.0 μmole的 p-硝基酚。

取代透過

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

10 - Combustible liquids


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Philip van der Zee et al.
Purinergic signalling (2018-05-13)
Recent research suggested an important role for pulmonary extracellular adenosine triphosphate (ATP) in the development of ventilation-induced lung injury. This injury is induced by mechanical deformation of alveolar epithelial cells, which in turn release ATP to the extracellular space. Measuring
Usefulness of salivary alpha amylase as a biomarker of chronic stress and stress related oral mucosal changes?a pilot study.
Vineetha R, et al.
Journal of Clinical and Experimental Dentistry, 6(2), e132-e132 (2014)
Natalie Chiu et al.
Food hydrocolloids, 69, 450-458 (2017-08-05)
Sodium (salt) was encapsulated within the inner water phase of w1/o/w2 food emulsions externally stabilised by starch particles with the ultimate aim of enhancing saltiness perception. The physical properties of the starch particles were modified by octenyl succinic anhydride (OSA)
High endogenous salivary amylase activity is associated with improved glycemic homeostasis following starch ingestion in adults.
Mandel A L, et al.
The Journal of Nutrition, 142(5), 853-858 (2012)
Tania Michelle Roberts et al.
Yeast (Chichester, England), 37(5-6), 336-347 (2020-02-18)
Saccharomyces cerevisiae cells grown in a small volume of chemically defined media neither reach the desired cell density nor grow at a fast enough rate to scale down the volume and increase the sample number of classical biochemical assays, as

Questions

1–4 of 4 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Hi There, is this ELISA Kit? many thanks, Ghaida.

    1 answer
    1. This kit is not an ELISA assay, as there are no antibodies involved. The principle of this kit is that alpha-Amylase cleaves the substrate ethylidene-pNP-G7 to produce smaller fragments that are eventually modified by alpha-glucosidase. P-nitrophenol is then released, which leads to the color development that is monitored by absorbance at 405 nm spectrophotometrically.

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  4. I want to know the name of each component of Amylase Activity Assay Kit

    1 answer
    1. The components of this kit are listed on the Product Datasheet which can be located in the 'DOCUMENTATION' section under 'More Documents':
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/377/793/mak009bul.pdf

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