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Merck

M8883

Sigma-Aldrich

4-甲基伞形酮磷酸酯

phosphatase substrate, fluorogenic, ≥98% (HPLC), powder

别名:

4-甲基伞形基磷酸

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100 MG
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100 MG
$122.00
250 MG
$204.00
1 G
$641.00
5 G
$2,480.00

About This Item

经验公式(希尔记法):
C10H9O6P
CAS号:
分子量:
256.15
Beilstein:
1687229
EC 号:
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.32

$122.00


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新价格,新优惠!

产品名称

4-甲基伞形酮磷酸酯, phosphatase substrate

描述

phosphatase substrate

方案

≥98% (HPLC)

表单

powder

溶解性

water: 25 mg/mL, clear to very slightly hazy, colorless

荧光

λex 319 nm; λem 384 nm (pH 9.1)
λex 360 nm; λem 449 nm (Reaction product)

储存温度

−20°C

SMILES字符串

CC1=CC(=O)Oc2cc(OP(O)(O)=O)ccc12

InChI

1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)

InChI key

BCHIXGBGRHLSBE-UHFFFAOYSA-N

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应用

4-甲基伞形酮磷酸酯可在肽结合试验中作为碱性磷酸酶的作用底物。[1]也用作酶联免疫吸附测定(ELISA)的荧光底物。[2][3]

生化/生理作用

4-甲基伞形酮磷酸酯可作为钙调蛋白依赖性磷酸酶的荧光底物,也可用于碱性磷酸酶的动力学研究。在酶联免疫吸附测定(ELISA)中,可作为碱性磷酸酶作用底物[4]。在人免疫缺陷型病毒抗体的酶免疫分析中,作为底物的4-甲基伞形酮磷酸酯比酚酞单磷酸酯灵敏7倍,比对硝基苯磷酸酯灵敏8-13倍。

底物

磷酸酶的荧光底物。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Ryota Fujii et al.
Biochimica et biophysica acta, 1631(2), 197-205 (2003-03-14)
The fluorescent organophosphorus esters, diethyl 4-methylumbelliferyl phosphate (1), ethyl hexyl 4-methylumbelliferyl phosphate (2) and ethyl 4-methylumbelliferyl heptylphosphonate (3) have been synthesized and evaluated as a sensitive active-site titrant of lipase. The phosphorus esters 1, 2 and 3 inactivated the lipase
Emna Hannachi et al.
Biotechnology reports (Amsterdam, Netherlands), 23, e00360-e00360 (2019-07-26)
The purpose of this study was to implement a fluorometric method for enhancing the detection sensitivity of Toxoplasma gondii in biological fluids. To address this challenge, we designed and produced a recombinant immunoconjugate tool based on a single-chain antibody fragment
Andréa Toma et al.
Diabetes, 58(2), 394-402 (2008-11-18)
A restricted region of proinsulin located in the B chain and adjacent region of C-peptide has been shown to contain numerous candidate epitopes recognized by CD8(+) T-cells. Our objective is to characterize HLA class I-restricted epitopes located within the preproinsulin
Maya Heath et al.
JAMA network open, 2(11), e1914996-e1914996 (2019-11-09)
Necrotizing enterocolitis (NEC) in preterm infants is an often-fatal gastrointestinal tract emergency. A robust NEC biomarker that is not confounded by sepsis could improve bedside management, lead to lower morbidity and mortality, and permit patient selection in randomized clinical trials
Francesca Bertolini et al.
Journal of pharmaceutical sciences, 96(11), 2931-2944 (2007-08-21)
Oxidative damage to proteins, implicated amongst other in the etiology and progression of Parkinson's disease (PD) and Alzheimer's disease (AD), results in the loss of specific biological protein function. A simple, sensitive, and cost-effective fluorimetric test to assess the antioxidant

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