Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
M2933
MES 水合物
BioPerformance Certified, suitable for cell culture, ≥99.5%
别名:
2-吗啉基乙磺酸水合物, 2-(N-吗啉基)乙磺酸 水合物, 4-吗啉乙磺酸
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About This Item
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质量水平
等级
BioPerformance Certified
Molecular Biology
for molecular biology
方案
≥99.5%
表单
crystalline powder
储存条件
dry at room temperature
技术
cell culture | mammalian: suitable
immunofluorescence: suitable
nucleic acid detection: suitable
杂质
endotoxin and Total Aerobic Microbial Count, tested
颜色
white
有效pH范围
5.5-6.7
pKa
6.1
溶解性
water: 335.3 g/L at 20 °C
适用性
suitable for component for culture media
suitable for molecular biology
应用
agriculture
diagnostic assay manufacturing
life science and biopharma
sample preparation
异质活性
DNase, RNase, protease, none detected
SMILES字符串
O.OS(=O)(=O)CCN1CCOCC1
InChI
1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2
InChI key
MIIIXQJBDGSIKL-UHFFFAOYSA-N
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一般描述
除了这些应用以外，MES水合物还被广泛用于植物培养基、试剂溶液和生理实验中pH 值的调节。它是研究pH 值对酶促反应的作用以及考察蛋白质和其它生物分子与金属离子相互作用的首选试剂。作为Good′s缓冲液，MES 水合物满足以下严格标准：中值pKa，最大水溶性，在所有其他溶剂中的最小溶解度，最小盐效应，不同温度下的稳定性，化学和酶学稳定性，在可见光或紫外光谱范围的最小吸收，易于合成。此外，它不会与大多数金属离子形成复合物，从而确保涉及金属离子的应用得到可靠的结果。
应用
特点和优势
- 适用于分子生物学和细胞培养
- 可用作缓冲液成分，用于电泳和蛋白质分离
- 通过内毒素和好氧微生物总数测试
- 不含DNA酶、切开酶（NICKase）、RNA酶和蛋白酶
- 经测试证实重金属污染浓度低，从而确保其适用于各种应用
- pKa值为6.1 的(25 °C)可在pH 5.5-6.7 (25 °C) 范围内有效缓冲
- 易溶于水
- 金属离子结合能力极低
- 与其它缓冲液如Tris和磷酸盐相比细胞毒性更低
- 在很宽的pH范围内保持稳定
- 低紫外吸收率
- 反应性极低
制备说明
储存及稳定性
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
历史批次信息供参考:
分析证书(COA)
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相关内容
Discover high-purity biological buffers for production scale. Ensure stability, pH control, and consistent supply with our expert manufacturing solutions.
了解生产规模的高纯度生物学缓冲液通过我们专业制造溶液，确保稳定性、pH控制和稳定供应。
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Helpful?
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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What is the molar weight of this compound? What does the x stand for in its formula?
1 answer-
This product is the hydrated form of MES. The anhydrous molecular weight of MES is 195.24 g/mol. The maximum allowable water content of this material is 9%. This value varies from lot to lot and is reported in the product Certificate of Analysis. As an example, the x value and molecular weight of a lot reporting a Water content of 8% may be determined as follows:
(108%/100%) * 195.24 g/mol= 210.8592 g/mol
210.8592g/mol - 195.24g/mol = 15.6192g/mol
15.6192 g/mol/18.02 g/mol = 0.86677, which is approximately = 1.
Therefore, since the value of x = 1 in the formula, The "as is" Molecular Weight of the compound = 213.25 g/molHelpful?
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