If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf
选择尺寸
About This Item
推荐产品
方案
≥99% (titration)
表单
powder
储存条件
dry at room temperature
杂质
≤1.0% water
颜色
white
pH值（酸碱度）
2.5-4 (25 °C, 97.6 g/L)
有效pH范围
5.5-6.7
pKa
6.1
密度
1.23 g/cm3 at 20—25 °C
1.27 g/cm3 at 20—25 °C
应用
clinical research
diagnostic assay manufacturing
life science and biopharma (
)
metabolomics (
)
SMILES字符串
OS(=O)(=O)CCN1CCOCC1
InChI
1S/C6H13NO4S/c8-12(9,10)6-3-7-1-4-11-5-2-7/h1-6H2,(H,8,9,10)
InChI key
SXGZJKUKBWWHRA-UHFFFAOYSA-N
一般描述
这种缓冲液应用广泛，可用于在细胞培养基、基于蛋白的缓冲配方和电泳缓冲液中维持环境稳定。其重要性也见于抗体、多肽、蛋白质、血液成分和生长因子的纯化过程。MES缓冲液也是毛细管电色谱的良好选择，具有很低的离子迁移率。重要的是，它可作为二甲胂酸盐和非两性离子缓冲液（如柠檬酸盐和苹果酸盐）等有毒试剂的更安全的替代品。在各种实验环境中，MES缓冲液也用于精细调节生长培养基的pH值，稳定酶溶液，并可作为PAGE电泳缓冲液的成分。
应用
特点和优势
- 经检测确认重金属污染水平低，适合各种应用
- 有效缓冲范围pH 5.5-6.7 (25 °C)，pKa 6.1 (25 °C)
- 极易溶于水
- 金属离子结合性极弱
- 细胞毒性低于Tris和磷酸盐等缓冲液
- 大范围pH值内保持稳定
- 紫外吸收低，反应性极弱
质量
储存分类代码
11 - Combustible Solids
WGK
WGK 3
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
其他客户在看
实验方案
聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明
Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What does low moisture content mean in the description of Product M3671, MES - low moisture?
1 answer-
The amount of water/moisture for MES (M3671) is tested by Karl Fisher and meets a specification of less than 1%. The other MES Hydrates offered have a specification of less than 9%.
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Is Product M3671, MES - low moisture, autoclavable?
1 answer-
Autoclaving is not recommended for any sulfonic acid buffer solution. Sterilization of a MES buffer solution can be accomplished by filtering it through a 0.2 micron filter.
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What is the solubility of Product M3671, MES - low moisture?
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We test the solubility of Prod. No. M3671 at 20 grams in 80 mL of water. A clear, colorless solution is obtained. This concentration is not necessarily the upper solubility limit of MES in water.
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How does one prepare a buffer using Product M3671, MES - low moisture, free acid?
1 answer-
A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH. Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH.
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What is the pH of a solution of Product M3671, MES - low moisture?
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The pH of a 0.5 M solution in water should be between 2.5 and 4.0.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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