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Merck

D141

Sigma-Aldrich

毛地黄皂苷

Used as non-ionic detergent

别名:

Digitin

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100 MG
$203.00
500 MG
$655.00

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100 MG
$203.00
500 MG
$655.00

About This Item

经验公式(希尔记法):
C56H92O29
CAS号:
分子量:
1229.31
Beilstein:
78654
EC號碼:
MDL號碼:
分類程式碼代碼:
12161900
PubChem物質ID:
NACRES:
NA.25

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生物源

plant seeds (Digitalis purpurea)

品質等級

形狀

powder

光學活性

[α]20/D −54°, c = 2.8 in methanol(lit.)

分子量

micellar avg mol wt 70,000

凝集数

60

雜質

<6.0% water

CMC

<0.5 mM (20-25°C)
<0.5 mM (20-25°C)

mp

230-240 °C (dec.) (lit.)

溶解度

H2O: ~5 % (w/v) (solubilized by heating to 95 °C - 98 °C and then cooling to room temp.)

SMILES 字串

C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]34CC[C@@H](C)CO4)([H])[C@@H](O)[C@@]1([H])[C@@](CC[C@]5([H])[C@@]6(C[C@@H](O)[C@H](O[C@]([C@@H]([C@@H](O)[C@H]7O[C@@](O[C@H](CO)[C@@H](O)[C@@H]8O[C@@](OC[C@@H](O)[C@@H]9O)([H])[C@@H]9O)([H])[C@@H]8O[C@@](O[C@H](CO)[C@H

InChI

1S/C56H92O29/c1-19-7-10-56(75-17-19)20(2)31-45(85-56)37(67)32-22-6-5-21-11-26(24(61)12-55(21,4)23(22)8-9-54(31,32)3)76-50-42(72)39(69)44(30(16-60)80-50)81-53-48(47(36(66)29(15-59)79-53)83-49-40(70)33(63)25(62)18-74-49)84-52-43(73)46(35(65)28(14-58)78-52)82-51-41(71)38(68)34(64)27(13-57)77-51/h19-53,57-73H,5-18H2,1-4H3/t19-,20+,21+,22-,23+,24-,25-,26-,27-,28-,29-,30-,31+,32-,33+,34-,35+,36-,37+,38+,39-,40-,41-,42-,43-,44+,45-,46+,47+,48-,49+,50-,51+,52+,53+,54-,55+,56-/m1/s1

InChI 密鑰

UVYVLBIGDKGWPX-KUAJCENISA-N

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一般說明

洋地黄皂苷是从紫色毛地黄植物(毛地黄)中提取的一种甾体皂苷,是一种多功能的非离子洗涤剂,在细胞生物学和生化研究中至关重要。其独特的属性使其成为探索细胞膜、膜蛋白和细胞内成分的宝贵工具。洋地黄皂苷′的关键特性和应用包括其能够有效溶解膜结合蛋白,同时保留其天然构象,使其成为研究膜蛋白相互作用和活性的理想工具。

此外,洋地黄皂苷选择性地透化富含胆固醇的质膜,并使细胞器膜保持完整。这一功能有利于特定细胞器的提取和纯化,有助于研究它们在细胞过程中的功能和作用。去垢剂′具有透化不同细胞类型的能力,可以通过将分子、抗体或酶引入细胞来研究细胞内成分和过程。此外,洋地黄皂苷还可用于免疫细胞化学实验,使得能够对细胞内蛋白质和结构进行标记和检测。

應用

洋地黄皂苷已与透化缓冲液一起用于细胞的环磷酸鸟苷-单磷酸腺苷(环GMP-AMP)刺激。
洋地黄皂苷已用于一项对从腹水肿瘤细胞中分离线粒体过程进行评估的研究中。 [1] 它还用于研究其对癌细胞中植物次生代谢物的细胞毒性的影响。 [2]

生化/生理作用

洋地黄皂苷在渗透细胞膜中起着关键作用。它能够裂解真核细胞,但不能裂解细菌膜。也可用于溶解细胞膜组分。它通过与细胞膜中的胆固醇相互作用形成孔隙。它因其温和的去污特性成为生物技术领域的首选。这种细胞膜活性化合物联合阿霉素治疗多药耐药癌细胞。

特點和優勢

适用于细胞生物学和生化研究的高度通用化合物

品質

制备不包括不溶于水的成分。

其他說明

如需了解生化试剂系列的更多信息,请填写此表

同類產品

产品编号
说明
价格

象形圖

Skull and crossbonesHealth hazard

訊號詞

Danger

危險聲明

危險分類

Acute Tox. 3 Oral - STOT RE 2

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Slide 1 of 3

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R W Moreadith et al.
Analytical biochemistry, 137(2), 360-367 (1984-03-01)
A new, improved procedure for isolating mitochondria from ascites tumor cells is described. The unique feature of this technique is the use of digitonin to make the cells susceptible to disruption by Teflon pestle/glass vessel homogenization. The yield and respiratory
Wattamon Srisakuldee et al.
Cardiovascular research, 103(1), 72-80 (2014-03-22)
Fibroblast growth factor 2 (FGF-2) protects the heart from ischaemia- and reperfusion-induced cell death by a mechanism linked to protein kinase C (PKC)ε-mediated connexin 43 (Cx43) phosphorylation. Cx43 localizes predominantly to gap junctions, but has also been detected at subsarcolemmal
Dominic S Alonzi et al.
Cellular and molecular life sciences : CMLS, 70(15), 2799-2814 (2013-03-19)
Endoplasmic reticulum-associated degradation (ERAD) is a key cellular process whereby misfolded proteins are removed from the endoplasmic reticulum (ER) for subsequent degradation by the ubiquitin/proteasome system. In the present work, analysis of the released, free oligosaccharides (FOS) derived from all
Xianying A Cui et al.
Journal of visualized experiments : JoVE, (70)(70), e50066-e50066 (2012-12-29)
In eukaryotes, most of the messenger RNAs (mRNAs) that encode secreted and membrane proteins are localized to the surface of the endoplasmic reticulum (ER). However, the visualization of these mRNAs can be challenging. This is especially true when only a
Xianying A Cui et al.
PLoS biology, 10(5), e1001336-e1001336 (2012-06-09)
In metazoans, the majority of mRNAs coding for secreted and membrane-bound proteins are translated on the surface of the endoplasmic reticulum (ER). Although the targeting of these transcripts to the surface of the ER can be mediated by the translation

实验方案

This page shows how to solubilize membrane proteins with products from Cytiva.

相关内容

This page shows how to solubilize membrane proteins with products from GE Healthcare.

Questions

1–9 of 9 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the stability of D141 and N7537 in the stock solution in DMSO?

    1 answer
    1. D141 can remain stable for up to one week when refrigerated, whether in water or DMSO. On the other hand, N7537 can be stored in DMSO (or ethanol) at -20°C for up to two weeks.

      Helpful?

  4. Wie kann ich das Produkt vor den Experiment aufreinigen? haben Sie ein Protokoll dazu? danke Beste Grüße Ayoub

    1 answer
    1. There is no specific protocol for the purification of item D141 available. The quality of this product is already detailed as being free of water-insoluble constituents. The purpose of the purification would be to remove these water-insoluble impurities.
      This is usually done through a recrystallization technique. This product will dissolve at 1 g in 20 mL of water at 95 - 98°C, as detailed on the corresponding specification sheet:
      https://www.sigmaaldrich.com/specification-sheet/SIGMA/D141

      Thus, it should be dissolved directly in water with high heat without any prior purification.

      Helpful?

  5. What is the storage condition for digitonin ?

    1 answer
    1. This product should be stored in room temperature. Please keep the container tightly closed in a dry and well-ventilated place. To view safety handling information, please view the safety data sheet for the product:
      https://www.sigmaaldrich.com/sds/sigma/d141

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. What is the solution stability of Product D141, Digitonin?

    1 answer
    1. We recommend making solutions fresh for use.

      Helpful?

  8. How pure is Product D141, Digitonin?

    1 answer
    1. The purity of this product is evaluated by Thin Layer Chromatography. The actual results are lot specific and will be reported on the Certificate of Analysis of each lot.

      Helpful?

  9. What is the solubility of Product D141, Digitonin?

    1 answer
    1. The solubility of D141 is tested by heating a 5% solution in water to 95-98°C and then cooling the solution to room temperature. After cooling, the solution should remain practically clear for at least 24 hours. The product can also be dissolved at 100mg/4mL of warm ethanol.

      Helpful?

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