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Merck

H8889

Sigma-Aldrich

Histopaque®-1077 Hybri-Max

liquid, sterile-filtered, BioReagent, suitable for hybridoma

别名:

Histopaque 1077

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100 ML
$111.00
500 ML
$565.00

$111.00


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100 ML
$111.00
500 ML
$565.00

About This Item

UNSPSC代码:
12352201
NACRES:
NA.75

$111.00


请联系客服了解存货情况
新价格,新优惠!

获取大包装报价

无菌性

sterile-filtered

产品线

BioReagent

表单

liquid

技术

cell culture | hybridoma: suitable
single cell analysis: suitable

杂质

endotoxin, tested

密度

1.077 at 25 °C (relative density)(lit.)

储存温度

2-8°C

一般描述

Histopaque®-1077是包含多蔗糖和泛影酸钠的溶液,密度调整到1.077 +/- 0.001 g/cm3[1]这一培养基帮助来自小体积全血的活的淋巴细胞快速恢复。这一技术适合于调节淋巴溶解和人类淋巴细胞抗原(HLA)2分型的细胞。它还可用作T-、B-和“空”淋巴细胞计数前的起始分离步骤。用来建立密度培养基,用于淋巴细胞和其他单核细胞的纯化。

应用

Histopaque®-1077 Hybri-Max已用于分离:
  • 外周血单个核细胞(PBMC)与肝素化血,梯度离心法[2]
  • 来自外周血的免疫细胞(淋巴细胞)[3]
  • PBMC与血液[4]

法律信息

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

本疑难排查指南针对使用 Histopaque 时观察到的最常见错误来源提出解决方案,但这并不代表本指南是全面无遗的。

相关内容

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

Questions

1–2 of 2 Questions  
  1. What is the formulation for H8889 Histopaque-1077 Hybri-Max?

    1 answer
    1. The formulation for H8889 Histopaque-1077 Hybri-Max consists of 5.7% polysucrose 400 and 9% sodium diatrizoate.

      Helpful?

  2. Is there a product in your inventory that is comparable to the Corning product 25-072-CV?

    1 answer
    1. Upon comparison of the formulation and product description, the comparable product from MilliporeSigma is identified as product number H8889. Both products, 25-072 and H8889, have a density of 1.077 g/ml and share the same formulation components, including Diatrizoic acid at 96.219 and Polysucrose 400 at 61.363.

      Helpful?

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