H1136 is not supplied in solution form. The product is lyophilized directly into the ampule and its appearance is a clear, thin film.
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1 AMP
$335.00
5 X 1 AMPS
$1,100.00
About This Item
CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.54
推荐产品
生物来源
Streptomyces sp. (S. hyalurolyticus)
质量水平
偶联物
conjugate (Glucosaminoglycan)
表单
film (Clear)
包装
ampule of 300 × units
储存温度
−20°C
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一般描述
应用
生化/生理作用
透明质酸裂解酶在β-D-GlcNAc-(1→4)-β-D-GlcA键上切割透明质酸，得到3-(4-脱氧β-D-葡萄糖-4-烯基糖基)-N-乙酰基-D-葡糖胺四糖和六糖。与其他透明质酸酶不同，该酶对透明质酸具有特异性，并且对软骨素和硫酸软骨素无活性。[4]
透明质酸酶属于内切糖苷酶，通过配合β-葡糖醛酸酶和β-N-乙酰氨基葡萄糖苷酶等外切糖苷酶完成其内切功能。透明质酸酶调节透明质酸的周转代谢。[1]
单位定义
在 37 ºC、pH 5.35 条件下，一单位每分钟可降解 0.75 μg 多糖透明质酸（通过比浊吸光度测量(λ: 600nm)，与 BSA 复合 45 分钟后测量）。
其他说明
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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商品
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
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实验方案
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
在测定透明质酸酶活性时，使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下，一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。
相关内容
A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.
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What is the volume in each vial/ampoule?
1 answer-
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You mention the following buffer composition for reconstitution: "...20 mM sodium phosphate buffer, with 77 mM sodium chloride, 0.01% BSA, pH 7.0". If I want to refreeze aliquots, would you suggest the same buffer? Isn't sodium phosphate harmful here?
1 answer-
Long-term solution stability studies have not been performed on the microbial source hyaluronidase. However, the bovine source products are stable for up to 1 month at 4 deg. C (500 mM acetate buffer pH 6.0) and several months at - 20 deg. C. Aliquot solutions to store frozen, avoid repeated freeze/thaw cycles. The recommended reconstitution buffer should include 0.01% BSA which acts as a stabilizer, there is no indication that the recommended sodium phosphate/sodium chloride buffer would be inappropriate for freezing.
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How is the activity of Product H1136, Hyaluronidase,determined?
1 answer-
Sigma assays the enzyme against a USP hyaluronidase reference standard as described in USP XXII-NF XVII, 644 (1990).
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I can't see any material in the container. Is material present in the container?
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The product is lyophilized directly into the ampule and its appearance is a clear, thin film.
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What amount of product is present in the vial of Product H1136, Hyaluronidase?
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The dry weight of the product is not determined. The vial should contain a minimum of 300 units of enzyme.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How should Product H1136, Hyaluronidase, be reconstituted?
1 answer-
Prior to determining the activity of this enzyme, we reconstitute it in 20 mM sodium phosphate buffer, with 77 mM sodium chloride, 0.01% BSA, pH 7.0.
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Are any other components present in Product H1136, Hyaluronidase, besides the enzyme?
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No protein or BSA has been added as a stabilizer. The enzyme was lyophilized from 0.5 mM Tris buffer, pH 7.0.
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What is the molecular weight of Product H1136, Hyaluronidase?
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The molecular weight for hyaluronidase from Streptomyces hyalurolyticus has been reported to be 91,000 DALTONS as determined by gel filtration.
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