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Merck
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H3757

Sigma-Aldrich

透明质酸酶 来源于牛睾丸

Type VIII, lyophilized powder, 300-1,000 U/mg

别名:

玻璃糖醛酸酶, 玻璃酸酶

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About This Item

CAS号:
37326-33-3
EC 号:
3.2.1.35 (BRENDA, IUBMB)
EC號碼:
253-464-3
MDL號碼:
MFCD00131351
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

bovine testis

品質等級

200

共軛

conjugate (Glucosaminoglycan)

種類

Type VIII

形狀

lyophilized powder

比活性

300-1,000 U/mg

分子量

~55 kDa (four subunits of 14 kDa each)

UniProt登錄號

Q5E985
Q8SQG8

相關疾病

cancer

儲存溫度

−20°C

基因資訊

cow ... HYAL1(515397) , HYAL2(281838)

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相关类别

生化／生理作用

这些酶可随机裂解透明质酸、软骨素和硫酸软骨素中的 β-N-乙酰己糖胺-[1→4] 糖苷键。
透明质酸酶可降解透明质酸，并已发现可在癌症进展期间会被不适当地调节。

單位定義

在 37 ºC、pH 5.35 条件下，一单位每分钟可降解 0.75 μg 多糖透明质酸（通过比浊吸光度测量(λ: 600nm)，与 BSA 复合 45 分钟后测量）。

準備報告

由无菌灌装的I-S型溶液制备。

其他說明

有关复合碳水化合物分的更多信息，请见www.sigma-aldrich.com/enzymeexplorer

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H334

防範說明

P261 - P284 - P501

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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Chaemoon Lim et al.
Journal of cancer prevention, 27(2), 122-128 (2022-07-23)
Osteosarcoma is the most frequent primary malignant bone tumor with higher incidences in children and adolescents. Despite clinical evolutions, patients with osteosacoma have had a poor prognosis. There has been increasing evidence that cancer is a stem cell disease. This
Kohtaro Miyazawa et al.
Histochemistry and cell biology, 133(1), 125-134 (2009-10-16)
Membranous epithelial cells (M cells) of the follicle-associated epithelium in Peyer's patches have a high capacity for transcytosis of several viruses and microorganisms. Here, we report that we have successfully established a bovine intestinal epithelial cell line (BIE cells) and
C Rollo et al.
Reproduction (Cambridge, England), 154(4), 375-385 (2017-09-08)
Acetylation of histone proteins is a major determinant of chromatin structure and function. Fertilisation triggers a round of chromatin remodelling that prepares the genome for the first round of transcription from the new embryonic genome. In this study we confirm
Takayuki Yamochi et al.
Journal of assisted reproduction and genetics, 38(10), 2591-2599 (2021-05-11)
To enhance the in vitro growth of porcine oocytes, we studied the effect of mural granulosa cells (MGCs) on the viability of oocytes attached to granulosa cells (oocyte-granulosa cell complexes, OGCs) that were obtained from early antral follicles. When OGCs
Lauren E Hamilton et al.
International journal of molecular sciences, 22(15) (2021-08-08)
The perinuclear theca (PT) of the eutherian sperm head is a cytoskeletal-like structure that houses proteins involved in important cellular processes during spermiogenesis and fertilization. Building upon our novel discovery of non-nuclear histones in the bovine PT, we sought to
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实验方案

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

透明质酸酶的酶法测定 (3.2.1.35)

在测定透明质酸酶活性时，使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下，一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。

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