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GE17-5279-02

Glutathione Seph High Performance

Cytiva 17-5279-02, pack of 100 mL

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About This Item

分類程式碼代碼:
23151817
NACRES:
NA.56
产品GE17-5279-02监管中，请联系销售询价或购买 联系技术服务部门

ligand

glutathione

包裝

pack of 100 mL

製造商／商標名

Cytiva 17-5279-02

基質

highly cross-linked 6% agarose

樣品平均尺寸

34 μm

cleaning

3-12

工作範圍

3-12

容量

>7 mg/mL binding capacity(medium)

相关类别

一般說明

Glutathione Sepharose™ High Performance is an affinity medium for high resolution purification of Glutathione S transferase (GST) tagged proteins.

應用

Glutathione Sepharose™ High Performance is used for the purification of GST-tagged Proteins from bacterial lysates and other S-transferases or glutathione-dependent Proteins. GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function.

特點和優勢

  • 34 μm bead size for improved efficiency and resolution
  • Fast, one-step purification of GST-tagged Proteins and other glutathione S-trasferase or glutathione-dependent Proteins
  • Mild conditions during the purification process preserves Protein antigenicity and function

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

General Considerations for Purification of GST-tagged Proteins

This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

Purification of GST-tagged Protein

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using Cytiva products.

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.

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实验方案

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

Purification Using GST HiTrap® 1 mL and 5 mL Columns: GSTrap™ 4B, GSTrap™ FF, and GSTrap™ HP

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

GSTrap™ Columns for Purification

GSTrap™ affinity columns facilitate protein purification with Glutathione Sepharose® chromatography media for efficient purification processes.

Characteristics of Glutathione Sepharose® Products

Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.

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