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Merck

E5529

Millipore

红色链霉亲和凝胶

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

形狀

suspension

儲存期限

1 yr

技術

western blot: suitable

基質

4% agarose

pH值

7.2

容量

~10 μg(biotin per ml of packed gel)

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

EZ View红色链霉亲和素亲和凝胶是由链霉亲和素共价连接到:
溴化氰活化的4%琼脂糖珠。 旨在捕获(下拉)生物素化的目标分子,例如蛋白质、肽、抗体、核酸、凝集素、受体和配体。

應用

当进行小规模的亲和力捕获(例如免疫沉淀)时,亲和基质很难在微量离心管中看到。 意外吸入树脂会导致结果的定量变化。 EZview红色亲和凝胶大大降低了沉淀损失的风险。 EZview树脂的性能与传统的非彩色亲和凝胶一样,但使得用户可以轻松区分沉淀物与上清液。这与更准确的数据相关,因为损失的蛋白质更少。
适用于免疫沉淀、蛋白质印迹和酶分析。

特點和優勢

  • 可见性增加-红色可降低意外吸入的风险
  • 通过减少意外损失提高目标蛋白的回收率
  • 重现性更高-收率更一致

外觀

含有50%甘油和15 ppm Kathon的PBS的1:1 (v/v)悬液。

法律資訊

EZview is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Alicia M Bostwick et al.
Biochemical and biophysical research communications, 528(3), 580-585 (2020-06-09)
Mammalian cells contain genetic information in two compartments, the nucleus and the mitochondria. Mitochondrial gene expression must be coordinated with nuclear gene expression to respond to cellular energetic needs. To gain insight into the coordination between the nucleus and mitochondria
Suet Yin Sarah Fung et al.
Current biology : CB, 27(1), 78-86 (2016-12-13)
After cleavage furrow ingression during cytokinesis, nascent daughter cells remain connected by an intercellular bridge (ICB) and the midbody [1, 2]. The midbody becomes an assembly platform for ESCRT complexes that split apart the plasma membrane (PM) anchored to the
Katarzyna M Zientara-Rytter et al.
Cells, 11(1) (2022-01-12)
Pex11, an abundant peroxisomal membrane protein (PMP), is required for division of peroxisomes and is robustly imported to peroxisomal membranes. We present a comprehensive analysis of how the Pichia pastoris Pex11 is recognized and chaperoned by Pex19, targeted to peroxisome
Ting-Yu Chang et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 138, 111485-111485 (2021-03-20)
Aberrant alteration of epigenetic information disturbs chromatin structure and gene function, thereby facilitating cancer development. Several drugs targeting histone deacetylases (HDACs), a group of epigenetic enzymes, have been approved for treating hematologic malignancies in the clinic. However, patients who suffer
Iram Khan Iqbal et al.
Autophagy, 17(11), 3511-3529 (2021-01-19)
The deacetylase SIRT1 (sirtuin 1) has emerged as a major regulator of nucleocytoplasmic distribution of macroautophagy/autophagy marker MAP1LC3/LC3 (microtubule-associated protein 1 light chain 3). Activation of SIRT1 leads to the deacetylation of LC3 and its translocation from the nucleus into

相关内容

利用亲和力或GST沉降、串联亲和纯化(TAP)和免疫共沉淀法研究体外蛋白-蛋白相互作用所需的pull-down检测方法、试剂和实验方案。

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

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