The specific concentration of non-ionic detergent for cleaning Sephadex Media is not explicitly mentioned in the available data. However, various sources suggest concentrations ranging from 0.1 – 0.5%. This information has not been validated. The general cleaning recommendations are as follows:
1. Wash with two column volumes of 0.2 M NaOH or a solution of a nonionic detergent. Washing the more porous Sephadex types (G-50 – G-200) with NaOH should be done outside the column because the gel will swell.
2. Re-equilibrate the gel with 2–3 column volumes of buffer before your next experiment. When necessary, the gel can be removed from the column and sterilized by autoclaving at 120 °C, pH 7.
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10 G
$214.00
50 G
$724.00
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10 G
$214.00
50 G
$724.00
About This Item
$214.00
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品質等級
技術
affinity chromatography: suitable
基質活性組
phase
膨潤
1 g swells to 9-11 mL
珠尺寸
20-80 μm
應用
life science and biopharma
相容性
Cytiva
一般說明
Sephadex G-50（细颗粒）葡聚糖凝胶是广泛使用的凝胶过滤树脂，设计用于分子量30,000以上生物分子的脱盐和缓冲液置换。因其胶珠较小而具有高效率。不同类型的Sephadex的交联度不同，因此其溶胀程度和分子分离范围也不同。Sephadex G-50属于G型，G型Sephadex的型号包括从小分子G-10到大分子G-75的五种型号。Sephadex G-50分为4种不同粒径，包括粗(Coarse)、中(Medium)、细(Fine)和超细(Superfine)颗粒。
Sephadex® G-50是一种用于亲和层析、蛋白质层析和凝胶过滤层析的凝胶过滤介质。Sephadex 是一种由葡聚糖和环氧氯丙烷交联而成的珠状凝胶。
分馏范围（MW）
球状蛋白质：1,500-30,000
葡聚糖：500-10,000
分馏范围（MW）
球状蛋白质：1,500-30,000
葡聚糖：500-10,000
應用
Sephadex® G-50 适用于：
- 低分子量和高分子量分子的分离
- 亲和色谱法、蛋白质色谱法和凝胶过滤色谱法
特點和優勢
- 一步完成脱盐、除污染和置换新缓冲液。
- 适于通过凝胶过滤纯化DNA，除去小分子。
- 凝胶珠较小，扩散距离更短。
- 经典的凝胶过滤树脂。
- Sephadex 脱盐被认为优于透析，因为它节省时间，稀释系数低，即便使用少量样品也能恢复高活性
其他說明
G5080-100G′更新后的产品编号为 GE17-0042-01
G5080-500G′更新后的产品编号为 GE17-0042-02
G5080-500G′更新后的产品编号为 GE17-0042-02
法律資訊
Sephadex is a registered trademark of Cytiva
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Clinical-scale radiolabeling of a humanized anticarcinoembryonic antigen monoclonal antibody, hMN-14, with residualizing 131I for use in radioimmunotherapy
Govindan SV, et al.
Journal of Nuclear Medicine, 46(1), 153-159 (2005)
Samuel Lopez-Nieves et al.
The New phytologist, 217(2), 896-908 (2017-10-11)
Diverse natural products are synthesized in plants by specialized metabolic enzymes, which are often lineage-specific and derived from gene duplication followed by functional divergence. However, little is known about the contribution of primary metabolism to the evolution of specialized metabolic
Relaxation of tyrosine pathway regulation underlies the evolution of betalain pigmentation in Caryophyllales
Lopez-Nieves S, et al.
The New phytologist, 217, 896-908 (2018)
L Zhang et al.
The EMBO journal, 14(2), 313-320 (1995-01-16)
Heme is a prosthetic group for numerous enzymes, cytochromes and globins, and it binds tightly, sometimes covalently, to these proteins. Interestingly, heme also potentiates binding of the yeast transcriptional activator HAP1 to DNA and inhibits mitochondrial import of the mammalian
L M Weiner et al.
Journal of immunology (Baltimore, Md. : 1950), 151(5), 2877-2886 (1993-09-01)
Bispecific monoclonal antibodies (BsmAb) with specificity for tumor Ag and effector cell trigger molecules have been shown to redirect the cytotoxicity of several peripheral blood mononuclear cell populations against relevant tumor. The BsmAb, 2B1, binds to the extracellular domain of
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What concentration of non-ionic detergent should be used for cleaning of G50 resins
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Can I use this for spin columns?
1 answer-
Yes, this product can be used for spin columns. For a prepared version, please view the Roche Quick Spin columns. See the link below to review this product:
https://www.sigmaaldrich.com/product/roche/11274015001Helpful?
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How stable is Sephadex® G-50, Product G5080, after swelling?
1 answer-
Sephadex® is stable in water, salt solutions, organic and denaturing solvents. If necessary, the gel can be removed from the column and sterilized by autoclaving at 120 deg C, pH 7. The pH stability is limited to low strengths and short times at extremes of pH 2-10, particularly in the acid range. After any washes using solutions at extremes of acidic or basic pH, the Sephadex® should be washed and reequilibrated with buffer at neutral pH as promptly as possible.
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What is the best buffer to use with Sephadex® G-50, Product G5080?
1 answer-
Sephadex® G-50 is very flexible in the buffers that can be used with it. Essentially any common aqueous buffer can be used with Sephadex® G-50. It is advised to filter buffers through a 0.22 micron filter before use.
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What kind of separation can I expect from Sephadex® G-50, Product G5080?
1 answer-
As a general rule of thumb, Sephadex® G-50 is appropriate for the separation of molecules of ~30 kDa from molecules ~1.5 kDa
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What does "G-50" mean in Sephadex® G-50, Product G5080?
1 answer-
"G" stands for gel. "50" refers to the water regain of the gel, in this instance, 5.0 g water per g dry gel.
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How can I clean the Sephadex® G-50, Product G5080, after use?
1 answer-
To clean the gel, you can wash the gel with 2 column volumes (CV) of 0.2 M NaOH, outside of the column because the gel will swell in basic pH, or in a solution of non-ionic detergent. Then you can rinse with water and re-equilibrate with 2-3 CV of buffer. If the resin is to be stored (longer than ~24 hours), equilibrate the resin with buffer containing an antimicrobial such as 20% ethanol or 0.02% sodium azide.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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