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用途
kit sufficient for 50 reactions
品質等級
分析物化學類別
proteins (FLAG® Affinity Gels)
技術
immunoprecipitation (IP): suitable
運輸包裝
wet ice
儲存溫度
−20°C
一般說明
Sigma的FLAG免疫沉淀试剂盒可快速、高效地免疫沉淀和洗脱FLAG标记蛋白。免疫沉淀过程使用ANTI-FLAG M2亲和凝胶进行，它是一种与琼脂糖树脂共价连接的高特异性单克隆抗体。亲和树脂可使FLAG标记蛋白的结合高效进行，而无需预备步骤或校准。免疫沉淀后的FLAG标记蛋白可以在酸性条件下或使用竞争性FLAG肽，从树脂上高效地洗脱下来。免疫沉淀后的蛋白可用于电泳或其它活性检测方法，以检测它们的大小、翻译后修饰及相互作用。
應用
更多产品信息，请访问我们的FLAG®应用页面。
特點和優勢
- 使用高特异性ANTI-FLAG M2亲和凝胶
- 无预备步骤或校准
- 3X FLAG™肽通过直接竞争实现高效和温和的洗脱
其他說明
所有步骤在2-8°C进行。
法律資訊
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Warning
危險聲明
危險分類
Aquatic Chronic 3 - Eye Irrit. 2
儲存類別代碼
10 - Combustible liquids
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
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Oleuropein, a terpene-derived glycosylated secoiridoid biosynthesized exclusively by members of the Oleaceae family, is involved in a two-component defense system comprising a β-glucosidase that activates oleuropein into a toxic glutaraldehyde-like structure. Oleuropein and its deglycosylated derivatives have high pharmaceutical interest.
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Molecular cancer research : MCR, 13(3), 538-547 (2014-11-09)
Epithelial cell-cell contacts maintain normal glandular tissue homeostasis, and their breakage can trigger epithelial-to-mesenchymal transition (EMT), a fundamental step in the development of metastatic cancer. Despite the ability of C-type lectin domains (CTLD) to modulate cell-cell adhesion, it is not
Kuancan Liu et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 28(3), 901-913 (2020-01-29)
Esophageal squamous cell carcinoma (ESCC) is a predominant cancer type in developing countries such as China, where ESCC accounts for approximately 90% of esophageal malignancies. Lacking effective and targeted therapy contributes to the poor 5-year survival rate. Recent studies showed
Kalpana Kannan et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(11), E1272-E1277 (2015-03-04)
High-grade serous ovarian cancer (HGSC) is among the most lethal forms of cancer in women. Excessive genomic rearrangements, which are expected to create fusion oncogenes, are the hallmark of this cancer. Here we report a cancer-specific gene fusion between BCAM
Samer Swedan et al.
Journal of virology, 85(19), 10090-10100 (2011-07-29)
Human respiratory syncytial virus (RSV), a major cause of severe respiratory diseases, efficiently suppresses cellular innate immunity, represented by type I interferon (IFN), using its two unique nonstructural proteins, NS1 and NS2. In a search for their mechanism, NS1 was
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