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Merck
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M8823

Millipore

抗-FLAG® M2磁珠

affinity isolated antibody

别名:

单克隆抗-FLAG® M2 小鼠抗, FLAG®树脂，用于高通量, FLAG®磁性亲和树脂, Flag®亲和树脂, 抗 ddddk, 抗 dykddddk

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.32

共軛

magnetic beads

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

M2, monoclonal

形狀

suspension

儲存期限

2 yr at -20 °C

分析物化學類別

proteins

技術

affinity chromatography: suitable
immunoprecipitation (IP): suitable

珠尺寸

20-75 μm

基質

superparamagnetic iron impregnated 4% agarose bead, with an average diameter of 50 μm.

同型

IgG1

容量

≥0.6 mg/mL binding capacity

運輸包裝

wet ice

儲存溫度

−20°C

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相关类别

一般說明

抗-FLAG M2磁珠是可与抗-FLAG M2（小鼠单克隆）抗体结合的4％琼脂糖微珠。 该M2抗体可识别FLAG序列的N-末端、Met N-末端和C-末端。 这可实现对含有FLAG肽序列的融合蛋白的检测和捕获。

應用

适用免疫沉淀分析。

洗脱方法：FLAG®肽、甘氨酸(pH 3.5)、3x FLAG®肽

更多产品信息请访问我们的FLAG®应用页面。

特點和優勢

该磁性属性可实现：
- 极为快速的分离
- 显著被加速的操作，例如重复的洗涤
-以板式进行多个样本的处理
该可导致：
- 更为快速的实验
- 更好的可重复性
- 目标蛋白更为准确的定量

外觀

以含10mM磷酸钠、150mM 氯化钠（pH 7.4）、 0.02%（w/v）叠氮化钠（PBA/A）的50%甘油的50%悬浮液供应

其他說明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

法律資訊

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

FLAG™ Affinity Gels, FLAG™ tag, 3x FLAG™ tag, DYKDDDDK tag

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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K Kollmann et al.
Leukemia, 31(4), 934-944 (2016-10-16)
Most myeloproliferative neoplasm (MPN) patients lacking JAK2 mutations harbour somatic CALR mutations that are thought to activate cytokine signalling although the mechanism is unclear. To identify kinases important for survival of CALR-mutant cells, we developed a novel strategy (KISMET) that
Chanqiong Zhang et al.
Cancer biology & therapy, 20(9), 1213-1222 (2019-04-16)
It is verified that long non-coding RNAs (lncRNAs) play crucial roles in various cancers. LncRNA LEF1-AS1 is a reported oncogene in colorectal cancer and glioblastoma. In this study, we unveiled that LEF1-AS1 markedly increased in oral squamous cell carcinoma (OSCC)
Ephrem G Kassa et al.
PLoS pathogens, 15(6), e1007851-e1007851 (2019-06-27)
Enteropathogenic E. coli (EPEC) is an extracellular diarrheagenic human pathogen which infects the apical plasma membrane of the small intestinal enterocytes. EPEC utilizes a type III secretion system to translocate bacterial effector proteins into its epithelial hosts. This activity, which
Chao Wang et al.
Cell discovery, 2, 16006-16006 (2016-07-28)
The Hippo signaling pathway regulates tissue growth and organ size through controlling cell growth, proliferation and apoptosis. During these processes, the coactivator Yorkie partners with the transcription factor Scalloped to mediate Hippo pathway-regulated cellular functions. Here, we demonstrate that Taiman
Eva Madi Riising et al.
PloS one, 3(7), e2704-e2704 (2008-07-17)
The Polycomb Repressive Complex 2 (PRC2) functions as a transcriptional repressor through a mechanism that involves methylation of Histone H3 at lysine 27. The PRC2 complex activity is essential for cellular proliferation, development, and cell fate decisions. PRC2 target genes
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商品

ANTI-FLAG® M2 Magnetic Beads

The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.

ANTI-FLAG® M2磁珠

FLAG®表达系统是一种表达、纯化和检测重组融合蛋白的成熟方法。作为FLAG®的可靠供应商，Sigma®现提供FLAG标签抗体标记的免疫磁珠，用于免疫沉淀、蛋白纯化和蛋白互作研究。ANTI-FLAG® M2磁珠由ANTI-FLAG® M2鼠单克隆抗体与超顺磁铁包覆的4%琼脂糖微珠偶联而成，平均粒径50 µM。M2抗体可结合哺乳动物、细菌和植物提取物融合蛋白N端、Met-N端或C端的FLAG®肽。

Anti-FLAG® M2磁珠

比较anti-FLAG® M2磁珠的小规模FLAG®标签蛋白纯化的不同洗脱方法。

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