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F1141

Sigma-Aldrich

Bovine Fibronectin

from bovine plasma, liquid, suitable for cell culture

别名:

纤连蛋白 溶液 来源于人类成纤维细胞

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1 MG
$430.00
2 MG
$737.00
5 MG
$1,490.00

$430.00


预计发货时间2025年2月03日详情


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1 MG
$430.00
2 MG
$737.00
5 MG
$1,490.00

About This Item

EC號碼:
MDL號碼:
分類程式碼代碼:
12352202
eCl@ss:
42020141
NACRES:
NA.75

$430.00


预计发货时间2025年2月03日详情


获取大包装报价

产品名称

纤连蛋白牛血浆, solution, sterile-filtered, BioReagent, suitable for cell culture

生物源

bovine plasma

品質等級

無菌

sterile-filtered

產品線

BioReagent

形狀

solution

包裝

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

技術

cell culture | mammalian: suitable

表面覆盖率

1‑5 μg/cm2

UniProt登錄號

結合特異性

Peptide Source: Collagen

Peptide Source: Fibrinogen

運輸包裝

wet ice

儲存溫度

2-8°C

基因資訊

bovine ... FN1(280794)

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一般說明

纤连蛋白是一种普遍存在的细胞外基质蛋白。它是一种镶嵌蛋白,由三种的重复模块共有氨基酸序列组成。[1]纤连蛋白属于一类高分子量糖蛋白。[2]细胞纤连蛋白由成纤维细胞表达,血浆纤连蛋白通常由肝细胞释放。[1]

應用

纤连蛋白(FN)牛血浆可用于:
  • 纤连蛋白和吸附分析(ELISA(酶联免疫吸附测定))[3]
  • 跨孔迁移[4]
  • 纤连蛋白测定[5]

生化/生理作用

纤连蛋白参与细胞粘附、细胞骨架组织、致癌性转化、细胞迁移、吞噬、止血和胚胎分化。[2]

分析報告

采用免疫电泳法评估蛋白质的均一性。

其他說明

产品F1141,牛血浆纤连蛋白是pH 7.5的0.05 M TBS溶液。

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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FIBRONECTIN AUTOANTIBODIES
Autoantibodies, 221-251 (2007)
Enhanced fibronectin adsorption on carbon nanotube/poly (carbonate) urethane: independent role of surface nano-roughness and associated surface energy
Khang D, et al.
Biomaterials, 28(32), 4756-4768 (2007)
CCL2/CCR2 chemokine signaling coordinates survival and motility of breast cancer cells through Smad3 and p42/44MAPK dependent mechanisms
Fang W B, et al.
The Journal of biological chemistry, jbc-M112 (2012)
Tests to predict preeclampsia
Chesley's Hypertensive Disorders in Pregnancy, 221-251 (2015)
Role of subnano-, nano-and submicron-surface features on osteoblast differentiation of bone marrow mesenchymal stem cells
Khang D, et al.
Biomaterials, 33(26), 5997-6007 (2012)

商品

Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.

纤连蛋白(FN)是由肝细胞产生的多功能细胞外基质糖蛋白,以接近微摩尔的浓度循环,并与局部产生的细胞FN一起在细胞表面组装成胞外基质原纤维。

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

细胞外基质(ECM)是由细胞分泌并在组织中围绕在其周围。

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实验方案

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

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Questions

1–6 of 6 Questions  
  1. Hi, I bought a vial with 5mg of Fibronectin (F1141), but so far, I have not been able to find the concentration of this solution. Can you please help me with this info? Thanks a lot in advance!

    1 answer
    1. The concentration of this product is 1 mg/mL. This information is reported in the product Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/f1141#product-documentation

      Helpful?

  2. What is the procedure for coating culture surfaces with fibronectin?

    1 answer
    1. To coat culture surfaces: 1. Dilute fibronectin in sterile balanced salt solution and coat the culture surface (1-5 μg/cm2) with a minimal volume.  2. Allow to air dry for at least 45 minutes at room temperature.  Excess fibronectin may be removed by aspiration, but this is not necessary.

      Helpful?

  3. How long can I store the fibronectin solution?

    1 answer
    1. The product is good for at least a year at 2-8 °C if kept sterile.

      Helpful?

  4. How do I choose which fibronectin product to use to coat culture surfaces for cell growth?

    1 answer
    1. The species source for the fibronectin used when coating culture surfaces is not important.  It is the presence of the RGD binding site that is present in all fibronectins that promotes binding.

      Helpful?

  5. How long can I store fibronectin coated plates, etc?

    1 answer
    1. Fibronectin coated cultureware can be stored for 2-4 weeks at 2-8 °C in a closed sterile container, or in sterile sealable bags.

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  6. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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