The concentration of this product is 1 mg/mL. This information is reported in the product Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/f1141#product-documentation
推荐产品
产品名称
纤连蛋白牛血浆, solution, sterile-filtered, BioReagent, suitable for cell culture
生物源
bovine plasma
品質等級
無菌
sterile-filtered
產品線
BioReagent
形狀
solution
包裝
pkg of 1 mg
pkg of 2 mg
pkg of 5 mg
技術
cell culture | mammalian: suitable
表面覆盖率
1‑5 μg/cm2
UniProt登錄號
結合特異性
Peptide Source: Collagen
Peptide Source: Fibrinogen
運輸包裝
wet ice
儲存溫度
2-8°C
基因資訊
bovine ... FN1(280794)
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訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1 - Skin Sens. 1
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves
历史批次信息供参考:
分析证书(COA)
其他客户在看
商品
Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.
纤连蛋白(FN)是由肝细胞产生的多功能细胞外基质糖蛋白,以接近微摩尔的浓度循环,并与局部产生的细胞FN一起在细胞表面组装成胞外基质原纤维。
The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.
细胞外基质(ECM)是由细胞分泌并在组织中围绕在其周围。
实验方案
Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.
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Hi, I bought a vial with 5mg of Fibronectin (F1141), but so far, I have not been able to find the concentration of this solution. Can you please help me with this info? Thanks a lot in advance!
1 answer-
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What is the procedure for coating culture surfaces with fibronectin?
1 answer-
To coat culture surfaces: 1. Dilute fibronectin in sterile balanced salt solution and coat the culture surface (1-5 μg/cm2) with a minimal volume. 2. Allow to air dry for at least 45 minutes at room temperature. Excess fibronectin may be removed by aspiration, but this is not necessary.
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How long can I store the fibronectin solution?
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The product is good for at least a year at 2-8 °C if kept sterile.
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How do I choose which fibronectin product to use to coat culture surfaces for cell growth?
1 answer-
The species source for the fibronectin used when coating culture surfaces is not important. It is the presence of the RGD binding site that is present in all fibronectins that promotes binding.
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How long can I store fibronectin coated plates, etc?
1 answer-
Fibronectin coated cultureware can be stored for 2-4 weeks at 2-8 °C in a closed sterile container, or in sterile sealable bags.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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