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Merck
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主要文件

F1056

Sigma-Aldrich

Human Fibronectin

from human plasma, powder, suitable for cell culture

别名:

纤连蛋白 溶液 来源于人类成纤维细胞

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1 MG
$749.00
2 MG
$975.00
5 MG
$1,590.00

About This Item

EC 号:
289-149-2
MDL编号:
MFCD00131062
UNSPSC代码:
12352202
NACRES:
NA.75

$749.00

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产品名称

纤维连接蛋白人血浆, lyophilized powder, BioReagent, suitable for cell culture, ≥95% (SDS-PAGE)

生物来源

human plasma

质量水平

200

无菌性

sterile

产品线

BioReagent

方案

≥95% (SDS-PAGE)

表单

lyophilized powder

包装

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

技术

cell culture | mammalian: suitable

表面覆盖度

1‑5 μg/cm2

溶解性

H2O: 1 mg/mL at 37 °C

UniProt登记号

P02751

结合特异性

Peptide Source: Collagen

运输

dry ice

储存温度

−20°C

基因信息

human ... FN1(2335)

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相关类别

一般描述

纤连蛋白(Fibronectin)是一种高分子质量粘合糖蛋白，[1]主要存在于细胞外基质(ECM)，广泛分布于组织和血液中。FN基因高度保守，在人体中通过选择性剪接产生20种以上亚单位。比较有名的有细胞FN和血浆FN。[2]血浆FN主要在肝脏中合成，细胞FN仅占循环纤连蛋白的5%[3]，由各种细胞局部表达。[2]

应用

人血浆纤连蛋白用于：
  • 在神经元选择培养基包被组织培养表面，用于神经元分化[4]
  • 包被二维细胞培养器皿，用于iPSC和iPSC‑来源BMEC的表征[5]
  • 包被培养支持物用于内皮细胞培养[6]

生化/生理作用

纤连蛋白在生物过程中起重要作用。可作用于表面受体和其他主要ECM蛋白（如胶原蛋白）介导细胞信号转导。[2]纤连蛋白参与多种细胞功能，包括细胞粘附、细胞骨架组织、致癌性转化、细胞迁移、吞噬、止血和胚胎分化。[3]这种糖蛋白可结合整合素、胶原蛋白、纤维蛋白和糖胺聚糖(GAG)。[1]纤连蛋白在促进细胞周期进展和增殖中起重要作用。这种作用影响周期蛋白和即刻早期反应基因，与细胞分裂和生长相关过程有关。纤连蛋白也参与多种细胞的生存和抗凋亡保护，包括上皮细胞、内皮细胞和白细胞。在这些细胞中，FN是促进细胞生存和防止程序性细胞死亡的关键因子。[7]FN基因突变与FN肾小球病和脊柱干骺端发育不良症有关。[2]纤连蛋白在创伤愈合与细胞外基质(ECM)组装中起重要作用，适合组织工程应用。[1]

注意

复溶溶液应以工作等份在-20°C 或更低温度下储存。不推荐涡旋、过度搅拌或额外冻融重组纤连蛋白。

制备说明

本品从 0.05 MTris 缓冲盐水 (pH 7.5) 冻干。溶于水 (1 mg/mL) , 在 37°C 至少溶解 30 min。可能残留少量未溶解物质，但不会影响性能。通过免疫电泳评价蛋白的均一性，检测内毒素，并且检测源材料的 HIV、乙型肝炎和丙型肝炎呈阴性。

在包被培养表面，纤维连接蛋白应在无菌平衡无菌盐溶液中稀释，并以最小体积包被。表面应在室温下风干 45 min，并可在 2-8℃ 下储存 2-4 周。

免责声明

仅供研究使用。本品在法国的科研应用受到监管，包括用于进口和出口活动（法国《公共卫生法典》Public Health Code Article L 1211-1 paragraph 2）。如《公共卫生法典》所述，购买者（即最终用户）需要从法国研究部(France Ministry of Research)获得进口授权。订购此产品即代表您已确认获得合法的进口授权。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000437204
0000437204
0000377119
0000367707
0000355124

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访问文档库

Fibronectin
Fabrizio M and Angela S
Encyclopedia of Immunology, 909-913 (1998)
Artificial biomaterials for urological tissue engineering
Farhat WA and Geutjes, PJ
Journal of Separation Science (2009)
Vivek V Thacker et al.
eLife, 9 (2020-11-25)
We establish a murine lung-on-chip infection model and use time-lapse imaging to reveal the dynamics of host-Mycobacterium tuberculosis interactions at an air-liquid interface with a spatiotemporal resolution unattainable in animal models and to probe the direct role of pulmonary surfactant
Fibronectin
Breisnes HW, et al.
Biochemistry of Collagens, Laminins and Elastin (2024)
Jianfei He et al.
Redox biology, 36, 101631-101631 (2020-08-19)
Anastellin (AN), a fragment of the first type III module in fibronectin (FN), initiates formation of superfibronectin, a polymer which resembles the native cell-derived fibrillar FN found in the extracellular matrix of many tissues, but which displays remarkably different functional
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商品

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

细胞外基质蛋白质和细胞培养优化工具

细胞外基质蛋白质，如层粘连蛋白、胶原蛋白和纤连蛋白，可用作细胞培养中的细胞附着基底。

实验方案

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

Questions

1–10 of 11 Questions  

  1. What is the molecular weight of this product?

    1 answer

    1. Human fibronectin is composed of two nearly identical disulfide bound peptides. Each has a molecular weight of 220 kDa for a combined total of 440 kDa.

      Helpful?

  2. This is to ask a question regarding coating flasks with the human freeze-dried fibronectin (F1056). After dissolving it in sterile water and diluting to the desired concentration should I incubate the flasks at 4 or 37 degrees overnight? Many thanks!

    1 answer

    1. As noted in the DESCRIPTION section of the Product Detail Page, fibronectin should be diluted in a sterile balanced sterile salt solution and coated with minimal volume. The surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C. See the information below for a brief protocol:

      To coat culture surfaces:
      1. Dilute fibronectin in a sterile balanced salt solution
      and coat the culture surface (1-5 mg/cm2) with a
      minimal volume.
      2. Allow to air dry for at least 45 minutes at room
      temperature, Excess fibronectin may be removed
      by aspiration, but this is not necessary.
      3. Fibronectin coated cultureware can be stored for
      2-4 weeks at 2-8 °C in a closed sterile container or
      sterile sealable bags.

      Helpful?

  3. How do I solubilized Fibronectin?

    1 answer

    1. Dissolve at 1 mg/mL in water. Allow to dissolve for at least 30 minutes at 37 °C. A small amount of undissolved material may remain. This will not affect product performance.

      Helpful?

  4. How do I choose which fibronectin product to use to coat culture surfaces for cell growth?

    1 answer

    1. The species source for the fibronectin used when coating culture surfaces is not important.  It is the presence of the RGD binding site that is present in all fibronectins that promotes binding.

      Helpful?

  5. How long can I store the fibronectin solution?

    1 answer

    1. The product is good for at least a year at 2-8 °C if kept sterile.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. How do I determine how much fibronectin is in solution?

    1 answer

    1. As the product may contain undissolved material, the amount of fibronectin in solution can be determined by UV absorbance.  The extinction coefficient for a 1% solution (10 mg/ml) is 13.5 at 280 nm.

      Helpful?

  8. How do I test the wells/flasks that have been coated with fibronectin?

    1 answer

    1. We test the coated culture surface by culturing cells.  We compare the adherence of the cells to wells that have been coated with media containing BSA (negative) and media containing 10% FBS (positive).  The cell growth should be not less than 50% of the positive control and greater than 50% of the negative control.

      Helpful?

  9. What is the difference between Fibronectin Products F2006 and F1056?

    1 answer

    1. The difference between Product Nos. F2006 and F1056 is in the purity specifications and endotoxin testing.  Product  No. F2006 has a purity specification of ≥90% and is not tested for endotoxin contamination.  Product No. F1056 has a purity specification of ≥95% and is tested for endotoxin contamination with a specification of ≤100 EU/mg.

      Helpful?

  10. What is the procedure for coating culture surfaces with fibronectin?

    1 answer

    1. To coat culture surfaces: 1. Dilute fibronectin in sterile balanced salt solution and coat the culture surface (1-5 μg/cm2) with a minimal volume.  2. Allow to air dry for at least 45 minutes at room temperature.  Excess fibronectin may be removed by aspiration, but this is not necessary.

      Helpful?

1–10 of 11 Questions  

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