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E3762

Sigma-Aldrich

pFLAG-CMV™−5a,b,c 表达载体

0.5 mg/mL

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About This Item

MDL编号:
MFCD03100886
UNSPSC代码:
12352200

标签

FLAG® tagged

等级

for molecular biology

表单

buffered aqueous solution

浓度

0.5 mg/mL

运输

dry ice

储存温度

−20°C

一般描述

p-FLAG-CMV-5a、p-FLAG-CMV-5b和p-FLAG-CMV-5c表达载体可用于表达哺乳动物细胞中C末端FLAG® 标记的蛋白质。 pFLAG-CMV-5 a，b，c表达载体可用作 大肠杆菌 和哺乳动物细胞的穿梭载体。这些载体可促进C末端FLAG标记的融合蛋白的细胞内表达。哺乳动物细胞中的瞬时表达是由CMV启动子调控区驱动的。SV40复制起点使得其在表达SV40大T抗原的哺乳动物细胞（例如COS细胞）中具有最高效的复制。a，b，c系列使得所有三个阅读框可覆盖多重克隆位点（MCS）中的每个限制性酶切位点。

载体图谱和序列

组分

  • p-FLAG-CMV-5a表达载体（20μg）
  • p-FLAG-CMV-5b表达载体（20μg）
  • p-FLAG-CMV-5c表达载体（20μg）
  • pFLAG-CMV-5a-BAP对照质粒（20μg）

法律信息

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

储存分类代码

12 - Non Combustible Liquids

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Katherine L Bates et al.
Genetics, 178(4), 1989-2002 (2008-04-24)
High baselines of transcription factor activities represent fundamental obstacles to regulated signaling. Here we show that in Drosophila, quenching of basal activator protein 1 (AP-1) transcription factor activity serves as a prerequisite to its tight spatial and temporal control by
Hiroshi Tanaka et al.
Molecular cancer research : MCR, 7(4), 557-569 (2009-04-18)
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF superfamily and has selective antitumor activity. Although TNF-alpha-induced intracellular signaling pathways have been well studied, TRAIL signaling is not fully understood. Here, we identified a novel TRAIL
Nadine Pollak et al.
The Journal of biological chemistry, 282(46), 33562-33571 (2007-09-15)
NAD kinases (NADKs) are vital, as they generate the cellular NADP pool. As opposed to three compartment-specific isoforms in plants and yeast, only a single NADK has been identified in mammals whose cytoplasmic localization we established by immunocytochemistry. To understand
Jinlong Wang et al.
Biochimica et biophysica acta. Gene regulatory mechanisms (2018-07-18)
KRAB-containing zinc finger proteins (KZNF) constitute the largest family of transcriptional regulators in humans and play critical roles in normal development and tumorigenesis. However, the function and mechanism of most KZNFs remain unclear. Here, we report that ZNF496, a KZNF

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