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Merck
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主要文件

P5368

Sigma-Aldrich

Phosphate buffered saline

powder, pH 7.4, BioPerformance Certified, for molecular biology

别名:

PBS

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About This Item

MDL號碼:
分類程式碼代碼:
12161700
eCl@ss:
32129211
NACRES:
NA.25

产品名称

Phosphate buffered saline, BioPerformance Certified, pH 7.4

等級

BioPerformance Certified
for molecular biology

品質等級

形狀

powder

儲存條件

(Keep container tightly closed in a dry and well-ventilated place.)

技術

immunofluorescence: suitable

雜質

BioBurden, tested
endotoxin, tested

顏色

white

pH值

7.4

溶解度

water: 9.07-10.0 g/L, clear, colorless

正離子痕跡

Fe: ≤10 ppm
heavy metals (as Pb): ≤5 ppm

適合性

suitable for SDS-PAGE
suitable for molecular biology

應用

diagnostic assay manufacturing
histology
sample preparation

異物活動

DNase, RNase, Protease, none detected

SMILES 字串

OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]

InChI

InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5

InChI 密鑰

LOKCTEFSRHRXRJ-UHFFFAOYSA-I

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應用


  • Rapid and zero-cost DNA extraction from soft-bodied insects for routine PCR-based applications.: 本研究使用磷酸盐缓冲盐水 (PBS) 开发了一种经济高效的昆虫 DNA 提取方法,促进了常规PCR在分子生物学中的应用(Jangra & Ghosh, 2022)。

  • Injectable amnion hydrogel-mediated delivery of adipose-derived stem cells for osteoarthritis treatment.: 利用PBS和可注射水凝胶制备和递送脂肪干细胞,揭示其在创新骨关节炎治疗中的应用潜力 (Bhattacharjee et al., 2022)。

  • Rapid and sensitive detection of SARS-CoV-2 infection using quantitative peptide enrichment LC-MS analysis.: 本研究采用 PBS 进行样品制备,基于一种新型 LC-MS 方法检测 SARS-CoV-2,提供了一种快速灵敏的诊断工具(Hober et al., 2021)。

包裝

锡箔袋包装

重構

当溶于1L蒸馏水或去离子水时,一小袋内容物将得到0.01 M磷酸盐缓冲盐水(NaCl 0.138 M; KCl - 0.0027 M);pH 7.4,25°C。

其他說明

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儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Petra J Roubos-van den Hil et al.
Veterinary microbiology, 202, 58-63 (2016-09-27)
Enterotoxigenic E. coli (ETEC), causing post-weaning diarrhoea, is a major problem in weaned piglets. Individual animal responses to ETEC infection show high variability in animal experiments. Two studies were designed to optimize the ETEC F4ac infection model in piglets by
Jinjin Sun et al.
Nanoscale research letters, 14(1), 248-248 (2019-07-26)
Herein, a magnetic graphene field-effect transistor biosensor was prepared through the transfer of a chemical vapor deposition graphene film onto a glass substrate to produce a sensing film and conductive channel. By fixing 1-pyrenebutanoic acid succinimidyl ester onto graphene film
Jesús Gilberto Arámburo-Galvez et al.
Nutrients, 10(7) (2018-07-18)
Most food allergy cases are associated with a limited group of allergens. This could be attributed to an increased ability of some foods to sensitize and trigger allergic reactions. However, there are no validated animal models to evaluate the sensitizing
Gary Burness et al.
Biology open, 7(4) (2018-04-06)
Female birds transfer maternally derived antibodies (matAb) to their nestlings, via the egg yolk. These antibodies are thought to provide passive protection, and allow nestlings to avoid the costs associated with mounting an innate immune response. To test whether there
Yixi Chen et al.
The European journal of neuroscience, 49(4), 510-524 (2018-11-26)
An emerging treatment for Parkinson's disease (PD) is cell replacement therapy. Authentic midbrain dopaminergic (mDA) neuronal precursors can be differentiated from human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs). These laboratory-generated mDA cells have been demonstrated

商品

Learn about Supel™ BioSPME C18 96-pin tool to prepare a clean sample with minimal matrix effects for an LC-MS/MS analysis from human plasma and its comparison with acetonitrile-based precipitation.

The application note describes a comparative analysis of plasma protein binding results for free/bound drugs using two different sample extraction steps, with and without agitation.

Learn about Supel™ BioSPME C18 high-throughput devices to determine plasma protein binding and decrease the sample preparation time to less than an hour.

Comparative analysis of Supel™ BioSPME 96-Pin device with a rapid equilibrium dialysis technique for accuracy of measured values, sample cleanliness, and workflow time in drug protein binding.

实验方案

Preparation for biodegradable nanoparticles and their use in transfection protocols .

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