This is 10 mM each and can be found under Description-> General Description: The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water.
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一般說明
應用
特點和優勢
- 每种dNTP的纯度:最低99%
- 方便配制;每50 μL PCR使用1 μL
- 等摩尔量的各种dNTP可减少移液步骤
- 最大限度地降低PCR污染风险
- UltraPure超纯dNTP可帮助最大限度地提高关键PCR反应的一致性和得率
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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实验方案
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Method for amplification of DNA from damaged DNA sources. Particularly useful for DNA extracted from old samples.
使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法(<1min), and results in higher yields and more specificity over standard PCR methods.
Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
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Is this 10 mM each, or 10 mM total? This info should be clearly stated on the label and on your site.
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Does Product No. D7295, Deoxynucleotide Mix contain dTTP or TTP?
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The product contains Thymidine 5´-triphosphate sodium salt. Thymidine has a deoxy sugar, so it is a deoxythymidine.
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What are the components to Product D7295, Deoxynucleotide Mix?
1 answer-
The D7295 contains 10mM of each dATP, dCTP, dGTP and TTP in molecular biology grade water. This is for a total concentration of 40 mM.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How much Product D7295, Deoxynucleotide Mix, is needed for a PCR reaction?
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One μl is sufficient for a standard 50 μl PCR reaction.
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