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品質等級
用途
sufficient for 100 tests
儲存期限
Kit is stable for 24 months when unopened.
檢測方法
colorimetric
運輸包裝
wet ice
儲存溫度
−20°C
一般說明
细胞谷胱甘肽过氧化物酶活性检测试剂盒用于对组织提取物中的谷胱甘肽过氧化物酶进行定量检测。本试剂盒基于谷胱甘肽（GSH）氧化后可生成氧化型谷胱甘肽（GSSG）。利用谷胱甘肽过氧化物酶（GPx）将GSH氧化成GSSG，生成的GSSG在谷胱甘肽还原酶（GR）的催化下再次生成GSH以及NADP+NAPDH氧化为NADP+的过程伴随340 nm处吸收光的减少。此处吸收值减少与样本中谷胱甘肽过氧化酶活性呈正相关。
適合性
适用于测定组织提取物中的谷胱甘肽过氧化物酶
原則
谷胱甘肽过氧化物酶细胞活性测定试剂盒用于测定组织提取物中的谷胱甘肽过氧化物酶。该测定基于将谷胱甘肽（GSH）氧化为氧化型谷胱甘肽（GSSG）。这一反应由GPx催化，并用谷胱甘肽还原酶和NADPH将GSSG再循环回GSH。由于该酶是偶联反应的限速因子，因此在NADPH氧化成NADP的过程中，可通过测定340nm处NADPH吸光度的降低，表明谷胱甘肽过氧化物酶的活性。
其他說明
本试剂盒通过了兔网织红细胞裂解液和谷胱甘肽过氧化物酶的测试。
仅试剂盒组分
产品编号
说明
- tert-Butyl hydroperoxide 1 mL
相關產品
訊號詞
Danger
危險分類
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Org. Perox. F - Skin Corr. 1C - Skin Sens. 1 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
5.2 - Organic peroxides and self-reacting hazardous materials
其他客户在看
International Committee for Standardization in Haematology: recommended methods for red-cell enzyme analysis.
E Beutler et al.
British journal of haematology, 35(2), 331-340 (1977-02-01)
Ian M Scott et al.
Pesticide biochemistry and physiology, 141, 9-17 (2017-09-16)
Plant elicitors can be biological or chemical-derived stimulators of jasmonic acid (JA) or salicylic acid (SA) pathways shown to prime the defenses in many crops. Examples of chemical elicitors of the JA and SA pathways include methyl-jasmonate and 1,2,3-benzothiadiazole-7-carbothioate (BTH
Diversity of glutathione peroxidases.
F Ursini et al.
Methods in enzymology, 252, 38-53 (1995-01-01)
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Red blood cells of favism patients with acute hemolytic crisis have markedly more superoxide dismutase (superoxide:superoxide oxidoreductase, EC 1.15.1.1) and less glutathione peroxidase (glutathione:hydrogenperoxide oxidoreductase, EC 1.11.1.9) than either normal controls, glucose-6-phosphate dehydrogenase-deficient subjects or favism patients outside hemolytic crisis.
Roxana Oana Cojocariu et al.
Brain sciences, 10(11) (2020-11-21)
Background and Objectives: Irritable bowel syndrome (IBS) is a well-known functional gastrointestinal (GI) disorder exhibiting a wide range of symptoms due to individual variability and multifactorial etiology. Stress exposure is a major risk factor for the development of IBS. Here
商品
氧化应激，在一部分上，是由多种细胞过程产生的活性氧介导，并由诸如酶清除剂或抗氧化剂调节剂等细胞抗氧化剂机制所控制的。自由基，如活性氧，会通过细胞引起细胞损伤。
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
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