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CS0410

Sigma-Aldrich

谷胱甘肽S-转移酶（GST）活性检测试剂盒

sufficient for 500 multiwell tests

别名:

GST 酶活性测定试剂盒

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1 KIT
$817.00

$817.00

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1 KIT
$817.00

About This Item

EC 号:
202-551-4
UNSPSC代码:
12161503
NACRES:
NA.84

$817.00

请联系客服了解存货情况
获取大包装报价

用途

sufficient for 500 multiwell tests

质量水平

200

运输

dry ice

储存温度

−20°C

基因信息

human ... GSTA4(2941) , GSTM1(2944)

相关类别

一般描述

谷胱甘肽S-转移酶 (GST) 检测试剂盒用于测定总GST的活性。适用于细胞和细菌裂解物、组织匀浆以及血浆
和红细胞裂解物样品。

应用

该谷胱甘肽S-转移酶检测试剂盒采用1-氯-2,4-二硝基苯（CDNB），适用于广泛的GST同工酶。基本原理是谷胱甘肽硫醇基团结合到CDNB底物上后，在340nm处的吸光度会增加。

生化/生理作用

谷胱甘肽S-转移酶 (GST)是在哺乳动物解除许多外源物毒素过程中发挥重要作用的一组酶。该酶可将谷胱甘肽的硫醇基团连接到亲电的外源物上，保护细胞免受毒物损害，进一步帮助细胞抵抗化合物诱变、致癌和毒性作用。GST活性广泛存在于植物、昆虫、酵母、细菌以及大多数哺乳动物组织中，尤其是在解毒过程中发挥关键作用的肝脏中。

仅试剂盒组分

产品编号
说明
  • Dulbecco's Phosphate Buffered Saline 100 mL
  • L-Glutathione Reduced 1 g
  • GST (Control) .1 mL
  • Sample Buffer 5 mL
  • Substrate (CDNB) 1.2 mL

警示用语：

Danger

危险分类

Acute Tox. 2 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

储存分类代码

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000423654
0000423654
0000212520
0000212520
0000126464

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T Lin et al.
Toxicology, 235(1-2), 1-10 (2007-04-10)
Glutathione (GSH) is one of the most important antioxidants in mammalian cells. It also plays an important role in chemical detoxification. Some evidence showed that polycyclic aromatic hydrocarbons, such as benzo[a]pyrene (B[a]P [50-32-8]), could increase GSH content as a defense
Daniel Scotcher et al.
Drug metabolism and disposition: the biological fate of chemicals, 45(5), 556-568 (2017-03-09)
In vitro-in vivo extrapolation of drug metabolism data obtained in enriched preparations of subcellular fractions rely on robust estimates of physiologically relevant scaling factors for the prediction of clearance in vivo. The purpose of the current study was to measure
S Melino et al.
Amino acids, 41(1), 103-112 (2010-03-10)
Natural organosulfur compounds (OSCs) have been shown to have chemopreventive effects and to suppress the proliferation of tumor cells in vitro through the induction of apoptosis. The biochemical mechanisms underlying the antitumorigenic and anti-proliferative effects of garlic-derived OSCs are not
Didier Techer et al.
Environmental toxicology, 32(1), 227-240 (2015-12-18)
Gallic and pelargonic acids are biologically derived substances receiving a growing interest as eco-friendly biocides with potential applications in freshwater system management. However, some data gaps remain to address their chronic ecotoxicity issue, particularly for fish. This work aimed at
Maria Sehlstedt et al.
Particle and fibre toxicology, 7, 21-21 (2010-08-24)
Biomass combustion contributes to the production of ambient particulate matter (PM) in rural environments as well as urban settings, but relatively little is known about the health effects of these emissions. The aim of this study was therefore to characterize
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Questions

1–5 of 5 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Is there a protocol available for preparing tissue for assay with the CS0410 Glutathione-S-Transferase (GST) Assay Kit?

    1 answer

    1. A specific tissue preparation protocol is not included with this kit. Product C3228, CelLytic MT reagent for mammalian tissue, is suitable. Please see the link below to review the product datasheet and protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/317/238/c3228bul.pdf

      Flash freezing the sample in liquid nitrogen and cold homogenization methods are also suitable for use with this kit. There are a number of methods available in published literature. Please see the link below for articles that may be helpful:
      https://scholar.google.com/scholar?q=tissue+lysate+protocol&hl=en&as_sdt=0&as_vis=1&oi=scholart

      Helpful?

  4. Is this suitable for detecting all of GST activity or is there for specific GST family for example GSTA or GSTM activity only?

    1 answer

    1. This product has not been evaluated for specificity to each GST isozyme. However, there is no evidence to suggest that this kit does not react to all GST isozymes.

      Helpful?

  5. ¿Cuál es el mejor protocolo para su utilización?

    1 answer

    1. The product datasheet is listed in the DOCUMENTATION section under 'More Documents'. Please see the links below to access the Product Datasheet:
      https://www.sigmaaldrich.com/product/sigma/cs0410#product-documentation
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/101/301/cs0410bul.pdf

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