As noted in the DESCRIPTION section of the product page, Collagen Type I is a very long, thin and abundant protein. It is a supercoiled right-helix of three left-handed polypeptide chains. These chains are composed of ~1040 amino acids, which are essentially repeats of three amino acids -(Gly-X-Y)n. Gly is glycine and X and Y can be any amino acids.
A defined molecular weight is not established, however when run on SDS page, multiple bands are observed at a range of 120 - 160 kDa. Please see the link below to the datasheet for this and other collagen products:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/133/320/c0543inf.pdf
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产品名称
胶原蛋白 来源于大鼠尾, Bornstein and Traub Type I, powder, BioReagent, suitable for cell culture
生物源
rat tail
品質等級
產品線
BioReagent
形狀
powder
分子量
120—160 kDa
包裝
glass bottle of 5 mg
技術
cell culture | mammalian: suitable
表面覆盖率
6‑10 μg/cm2
溶解度
soluble
NCBI登錄號
UniProt登錄號
結合特異性
Peptide Source: Fibrinogen
Peptide Source: Laminin
儲存溫度
2-8°C
基因資訊
rat ... Col1a1(29393)
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相关类别
一般說明
所有胶原蛋白分子均由以三螺旋构象排列的三条肽链组成，其一级结构主要以三个氨基酸作为重复基序，即每隔两个氨基酸是一个甘氨酸残基，其前面的两个位置通常为脯氨酸或4-羟基脯氨酸。I型胶原不同于其他胶原蛋白的方面为其低赖氨酸羟基化和低碳水化合物构成。Col1a1(胶原蛋白，I型，α1)或胶原蛋白是一种主要的人体结构蛋白，它以纤维的形式聚集。这是一种很长很薄的蛋白质，是人体中发现的最丰富的蛋白质。它是由三条左旋多肽链组成的超螺旋右螺旋结构。这些链由~1040个氨基酸组成，基本上是三个氨基酸-(Gly-X-Y)n的重复序列。Gly是甘氨酸，X和Y可以是任何氨基酸，但人体中通常分别是脯氨酸和羟脯氨酸。[1]
Product is clear to hazy colorless solution with a few insolubles at 1 mg/ml in water with 2 μl acetic acid (or 0.1 N acetic acid). The insolubles can be removed by settling or centrifugation.
應用
生化／生理作用
胶原蛋白是结缔组织的基本成分。一个中国家庭的研究表明COL1A1(胶原酶I型)突变与I型成骨不全有关。[8][8]胶原蛋白和软骨下骨转换能力下调有关，可作为骨关节炎的关节间隙狭窄和骨刺状态的标志物。[9]鼠尾胶原蛋白用于在组织培养板上铺上薄层包被，促进锚定依赖性细胞附着，推荐使用浓度为 6-10 μg/cm2。本品不能用于制作3-D凝胶。I型胶原常作为细胞培养的附着基质，与成肌细胞、脊髓神经节、肝细胞、胚胎肺、心脏外植体、成纤维细胞、内皮细胞、胰岛细胞一起在I型胶原的膜或凝胶上成功培养。I型胶原也可用于特发性肺纤维化(IPF)的研究，研究内质网应激IPF对肺成纤维细胞的影响。酸性溶液中的胶原蛋白可以产生三维支架，用于生物工程和细胞培养。
準備報告
产品为澄清至浑浊的无色溶液，以1 mg/ml 的浓度溶于含有2 μl 乙酸（或 0.1 N 乙酸）的水中时存在少量不溶物。通过沉降或离心可去除此不溶物。
其他說明
胶原蛋白被分为许多种结构和遗传性不同的类型。我们使用Bornstein和Traub提出的命名法。不要混淆Sigma分类标识和公认的胶原蛋白分类类型。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Shali Chen et al.
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Diabetic retinopathy entails proliferation of vascular endothelial cells (ECs) and unregulated angiogenesis. We have previously shown that ECs increase the expression of an embryonic variant of fibronectin (FN), called extra domain-B FN (ED-B FN) in response to high glucose. We
M A Fallah et al.
Biomicrofluidics, 7(4), 44124-44124 (2014-01-10)
The role of von Willebrand factor (VWF) as a shear stress activated platelet adhesive has been related to a coiled-elongated shape conformation. The forces dominating this transition have been suggested to be controlled by the proteins polymeric architecture. However, the
Peggy Romero et al.
BioTechniques, 47(6 Suppl), iii-viii (2010-01-01)
Current methods to assess neurodegradation in dorsal root ganglion cultures as a model for neurodegenerative diseases are imprecise and time-consuming. Here we describe two new methods to quantify neuroprotection in these cultures. The neurite quality index (NQI) builds upon earlier
H P Huinink et al.
Magnetic resonance in medicine, 59(6), 1282-1286 (2008-04-19)
A major application of molecular MR imaging is receptor mapping of cells lining blood vessels with targeted contrast agents. Since these agents accumulate at interfaces, knowledge of their influence on the relaxation process in this specific configuration is a prerequisite
K Makibayashi et al.
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OCT (22-oxa-calcitriol), a vitamin D analog, has been reported to show strong inhibitory effects on mesangial cell proliferation in vitro. In the present study, we report a study of the effect of OCT on anti-thy-1 glomerulonephritis. Both OCT and 1,25(OH)(2)D(3)
商品
细胞外基质（ECM）是由细胞分泌并在组织中围绕在其周围。
The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.
培养和表征CSC群体的癌症干细胞培养基、球状体培养板和癌症干细胞标志物。
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
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How much is the molecular weight (dalton)?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Why am I seeing some insolubles in my collagen solution?
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The solubility specifications for this product is clear to hazy colorless solution with a few insolubles at 1 mg/ml in water with 2 μl acetic acid (or 0.1 N acetic acid). The insolubles can be removed by settling or centrifugation.
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How can I sterilize collagen solutions?
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We recommend transferring the collagen solution to a glass bottle with a screw cap and carefully layering chloroform at the bottom. The amount of chloroform to use should be ~10% of the volume of collagen solution. DO NOT SHAKE OR STIR. Allow to stand overnight at 2-8 °C. Aseptically remove the top layer containing the collagen solution to a new bottle. We do not recommend sterilizing the collagen solution by membrane filtration. We have found substantial protein loss by this method.
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How can I make a 3-D collagen gel?
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Not all lots of C7661 are suitable for 3D gels. Product No. C4243 has been use-tested in this application. If C4243 is not available, we recommend that customers screen lots of C7661 to try to make 3D gels. We have found that collagen that has been sterilized by gamma-irradiation or by chloroform cannot be used to make 3D collagen gels. The following protocol can be used to make collagen gels: Materials required:Collagen gel solution 1.5 - 3 mg/mL 10X tissue culture medium containing phenol red Sodium bicarbonate or HEPES buffer Procedure: 1.Measure out 800 μL of collagen solution. 2.Add 100 μL of 10X medium (buffered with 1X sodium bicarbonate or HEPES) 3.Adjust pH with 1 N sodium hydroxide, if required (100 μl or less) 4.Add 10X medium to bring volume to 1 ml 5.Mix contents well. Solution should maintain red color to indicate physiological pH6. Dispense into wells to a depth of 1-2 mm (approx. 15 μL/well) 7.Transfer to 37 °C for 20-40 minutes 8.Examine for gel formation The above volumes represent quantities for use in 24 well plates. Volumes can be adjusted to accommodate any culture vessel.
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How are collagen solutions stored?
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Collagen solutions should be stored in the refrigerator at 2-8 °C for up to 1 year, unless it becomes contaminated.
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How long can collagen coated plates be stored?
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We make no recommendations about storage of plates after coating. Since several factors such as humidity may impact longevity of coated plates, customers should make their own determination as to whether plates can be stored and under which conditions. We offer Sigma Screen collagen coated plates (Product No. S3315) that indicates the following: For optimal performance, the unopened product should be stored in a dry place at 2-8 °C. The product may be stored at room temperature for up to three months. The product should not be exposed to temperatures above 50 °C.
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