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Merck
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主要文件

C2249

Sigma-Aldrich

Human Collagen Type I

Collagen Solution from human fibroblasts

from human fibroblasts, liquid, 3 mg/mL, suitable for cell culture

别名:

Collagen Solution, Fibroblast Collagen

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20 ML
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About This Item

UNSPSC代码:
12352202
NACRES:
NA.75

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产品名称

胶原蛋白溶液 来源于人类成纤维细胞, 3 mg/mL, sterile-filtered, BioReagent, suitable for cell culture

生物来源

human fibroblasts

质量水平

100

无菌性

sterile-filtered

产品线

BioReagent

表单

solution

包装

pkg of 20 mL

浓度

3 mg/mL

技术

cell culture | mammalian: suitable

表面覆盖度

6‑10 μg/cm2

结合特异性

Peptide Source: Collagen

Peptide Source: Elastin

异质活性

endotoxin ≤5.0 μmole/min-mg protein

运输

wet ice

储存温度

2-8°C

相关类别

一般描述

胶原蛋白占人类总蛋白的三分之一。[1]胶原原纤维是含胶原组织的基本成分。主要表达于细胞外基质。[1]I型胶原蛋白是皮肤、骨骼、肌腱和其他纤维结缔组织的主要组成部分。不同于其他胶原蛋白的方面为其低赖氨酸羟基化和低碳水化合物构成。I型胶原蛋白是由2条α1(I)链和1条alpha2(I)链组成的异源三聚体，在中性pH和37 °C下会自发形成三螺旋支架。纯化人胶原蛋白溶液是采用适当的细胞培养生产系统由分泌人细胞外基质(hECM)的新生人成纤维细胞生产。本品单体含量高。采用严格检测的人成纤维细胞体外制备培养物，并按照适用的cGMP标准生产工艺纯化。人成纤维细胞已经过大量测试，证明了其病毒和其他外来因子的安全性。通过固定的验证步骤确保灭活潜在的朊病毒和/或病毒污染物。

应用

胶原蛋白溶液（来自人成纤维细胞）用于

  • 作为包被溶液组分，制备人骨髓芯片。[2]
  • 成纤维细胞样滑膜细胞培养。[3]
  • 作为微通道包被溶液用于器官芯片培养[4]

生化/生理作用

属于胶原蛋白家族的蛋白质支撑并滋养身体组织，例如肌腱、软骨、皮肤和眼巩膜。[5]1型胶原蛋白是组织弹性所需的多功能结构材料，并维持组织的稳定性和强度。[6]胶原蛋白也存在于无脊椎动物的足丝纤维（yssus thread），其有助于表面附着。[6]胶原蛋白缺陷会影响动脉组织的生物力学性质。[7]
胶原蛋白是结缔组织的重要成分。针对一个中国家庭的研究表明，COL1A1（I型胶原酶）的突变与I型成骨不全症有关。[8][9] 胶原蛋白与骨的软骨下转换相关，并且可能作为确定骨关节炎中关节间隙变窄和骨赘状态的标记物。[10]

储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCS2330
MKCR3049
MKCQ9388
MKCS4885
MKCR6761

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Synovitis biomarkers: ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis
Kjelgaard PC, et al.
Biomarkers, 20(8), 547-556 (2015)
Collagen in arterial walls: biomechanical aspects
Collagen, 285-324 (2008)
On-chip recapitulation of clinical bone-marrow toxicities and patient-specific pathophysiology
Chou DB, et al.
Nature Biomedical Engineering, 4(4), 394?406-394?406 (2020)
Cecilie Kjelgaard-Petersen et al.
Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals, 20(8), 547-556 (2016-02-11)
Characterize biomarkers measuring extracellular matrix turnover of inflamed osteoarthritis synovium. Human primary fibroblast-like synoviocytes and synovial membrane explants (SMEs) treated with various cytokines and growth factors were assessed by C1M, C3M, and acMMP3 in the conditioned medium. TNFα significantly increased
Collagen: structure and mechanics, an introduction
Collagen, 1-13 (2008)
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商品

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

细胞外基质蛋白质和细胞培养优化工具

细胞外基质蛋白质，如层粘连蛋白、胶原蛋白和纤连蛋白，可用作细胞培养中的细胞附着基底。

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Questions

1–7 of 7 Questions  

  1. How did you steralize this collagen?

    1 answer

    1. As indicated above under 'PROPERTIES', this collagen solution is sterile-filtered.

      Helpful?

  2. Is this product suitable for 3D gel formation? How should it be neutralized? How quickly does it polymerize once neutralized? Is there a protocol available for 3D gel formation?

    1 answer

    1. Procedure for 3-D Gel Preparation:

      1. Mix 8 parts of chilled collagen solution with 1 part of
      10 X PBS (Catalog Number P5493 or P5368) or
      10 X culture medium. Cells may be added following
      this step.

      2. Adjust pH of mixture prepared in step 1 to 7.2–7.6.
      Use of 0.1 M NaOH (10-fold dilution of Catalog
      Number S2770) or 0.01 M HCl (100-fold dilution of
      Catalog Number H9892) is recommended. Monitor
      pH adjustment carefully with pH meter, phenol red,
      or pH paper.

      3. To prevent gelation, maintain temperature of the
      mixture at 2–8C. To form gel, warm to 37C. For
      best results allow 45 minutes to 1 hour for gel
      formation.

      4. The gels can be dried under a laminar flow hood.

      Helpful?

  3. Is the monomer a tropocollagen?

    1 answer

    1. The human collagen is secreted from fibroblast cells, which cleave the loose ends of the procollagen molecule, leaving what is considered tropocollagen with the telopeptide region intact. An enzyme step is utilized to cleave the telopeptide, resulting in Atelocollagen, which maintains its triple helical formation but without the telopeptide region.

      Helpful?

  4. What is the 2D Hydrogel protocol for Product No. C2249?

    1 answer

    1. There has been no internal protocol for C2249 however this paper describes protocol for 2D Collagen Gel. Hong H, Stegemann JP. 2D and 3D collagen and fibrin biopolymers promote specific ECM and integrin gene expression by vascular smooth muscle cells. J Biomater Sci Polym Ed. 2008;19(10):1279-93. doi: 10.1163/156856208786052380. PMID: 18854122; PMCID: PMC2731795.

      Helpful?

  5. Is this a monomer?

    1 answer

    1. This product is a mix of monomers, dimers, trimers, and oligomers, with some remaining collagen fragments.

      Helpful?

  6. What solvent is used for product C2249?

    1 answer

    1. The solvent for product C2249 is 13 mM HCl.

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  7. What is the acid, and concentration of acid, that the collagen is solubilized within?

    1 answer

    1. The acid used to prepare this product is HCl. The final concentration of the solvent is 13 mM.

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