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Merck

C7527

Sigma-Aldrich

氯化胆碱

BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98%

别名:

(2-羟乙基)三甲基氯化铵

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100 G
$111.00
500 G
$182.00
1 KG
$302.00

$111.00


预计发货时间2025年4月07日详情


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100 G
$111.00
500 G
$182.00
1 KG
$302.00

About This Item

线性分子式:
(CH3)3N(Cl)CH2CH2OH
CAS号:
分子量:
139.62
Beilstein:
3563126
EC 号:
MDL编号:
UNSPSC代码:
12352205
PubChem化学物质编号:
NACRES:
NA.75

$111.00


预计发货时间2025年4月07日详情


获取大包装报价

生物来源

synthetic (organic)

产品线

BioReagent

方案

≥98%

表单

crystals

分子量

Mw 139.62 g/mol

技术

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

颜色

white

pH值(酸碱度)

5.0-6.5 (25 °C, 140 g/L)

mp

302-305 °C (dec.) (lit.)

溶解性

H2O: 50 mg/mL

SMILES字符串

[Cl-].C[N+](C)(C)CCO

InChI

1S/C5H14NO.ClH/c1-6(2,3)4-5-7;/h7H,4-5H2,1-3H3;1H/q+1;/p-1

InChI key

SGMZJAMFUVOLNK-UHFFFAOYSA-M

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一般描述

氯化胆碱是一种季铵盐,与氢键供体(HBD)缔合。[1]它是许多重要生理功能必不可少的成分,用作营养添加剂。胆碱是甲基转移反应的甲基来源。[2]

应用

氯化胆碱已用于:
  • 用作胆碱乙酰基转移酶 (ChAT)缓冲液组分,用于培养人成神经细胞瘤(SK-N-SH)细胞中的胆碱吸收[3]
  • 生成胆碱标准曲线,以定量磷脂酶D (PLD)活性[4]
  • 用作解剖内海马脑切片的切片溶液组分[5]

底物

胆碱乙酰基转移酶底物

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Harvie P Portugaliza et al.
Scientific reports, 9(1), 14595-14595 (2019-10-12)
Transmission of malaria parasites from humans to mosquito vectors requires that some asexual parasites differentiate into sexual forms termed gametocytes. The balance between proliferation in the same host and conversion into transmission forms can be altered by the conditions of
Choline: an essential nutrient for public health
Zeisel SH and Da Costa KA
Nutr. Rev, 67(11), 615-623 (2009)
Apoptosis of bovine granulosa cells: Intracellular pathways and differentiation
Carou MC, et al.
Acta Histochemica, 119(5), 462-470 (2017)
Synthesis and characterization of choline chloride based binary mixtures
Abbas Q and Binder L
ECS Transactions, 33(7), 49-59 (2010)
Functional calcium imaging in developing cortical networks
Dawitz J, et al.
Journal of Visualized Experiments, 119(56), e3550-e3550 (2011)

Questions

1–3 of 3 Questions  
  1. Choline chloride will be melted in water for addition to the medium. Can you please tell me the proper storage method, such as -30°C freezing ay -4°C refrigeration?

    1 answer
    1. This product is soluble in water at 50 mg/mL. The storage and stability conditions for this product upon reconstitution have not been determined.

      Helpful?

  2. Hi, is it possible to thaw choline chloride in PBS instead of water and add it to the medium?

    1 answer
    1. This product is soluble in water, tested at 50 mg/mL. Solubility in PBS has not been determined in-house. Literature suggests that choline chloride can be prepared with PBS. For reference, see https://en-cdn.bio-protocol.org/pdf/Bio-protocol807.pdf?rel_link=YmlvLXByb3RvY29sLm9yZy9lbi9icGRldGFpbD9pZD04MDcmdHlwZT0w

      Helpful?

  3. Hi, could you please provide the product information sheet for Choline chloride.

    1 answer
    1. There is no specific product information sheet offered for Choline Chloride. Please see the PROPERTIES section of the product page for details. Links to a sample Certificate of Analysis and a Certificate of Origin are provided below:

      Certificate of Analysis
      https://www.sigmaaldrich.com/certificates/COFA/C7/C7527/C7527-100G________SLBB8921V_.pdf

      Certificate of Origin
      https://www.sigmaaldrich.com/US/en/coo/undefined/C7527/SLBB8921V

      Helpful?

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