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Merck
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C5914

Sigma-Aldrich

非酶细胞裂解液 1x

Prepared in phosphate buffered saline without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture

别名:

Dissociation Solution

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100 ML
$107.00

$107.00


国内现货,预计发货时间2025年4月07日详情


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100 ML
$107.00

About This Item

MDL编号:
UNSPSC代码:
12352204
NACRES:
NA.75

$107.00


国内现货,预计发货时间2025年4月07日详情


获取大包装报价

质量水平

无菌性

sterile-filtered

产品线

BioReagent

表单

liquid

浓度

1 ×

技术

cell culture | mammalian: suitable

pH值(酸碱度)

6.9-7.1

运输

wet ice

储存温度

2-8°C

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一般描述

在人间充质细胞中通过非酶方法进行的细胞分离有助于脱离并保持其功能,特别是细胞表面趋化因子受体的表达。[1]

应用

特殊的非酶配方,用于从塑料或玻璃表面温和地剥离贴壁细胞。
细胞解离液非酶1x已用于视网膜星形胶质细胞培养基、[2] U87胶质母细胞瘤肿瘤细胞[3]和心肌干细胞(CSC)的解离。[4]

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Ineke D C Jansen et al.
International journal of molecular sciences, 21(17) (2020-08-29)
Lysosome associated membrane proteins (LAMPs) are involved in several processes, among which is fusion of lysosomes with phagosomes. For the formation of multinucleated osteoclasts, the interaction between receptor activator of nuclear kappa β (RANK) and its ligand RANKL is essential.
Non-enzymatic dissociation of human mesenchymal stromal cells improves chemokine-dependent migration and maintains immunosuppressive function
Garg A, et al.
Cytotherapy, 16(4), 545-559 (2014)
Isolation and characterization of murine retinal astrocytes
Scheef E, et al.
Molecular Vision, 11(4), 613-624 (2005)
Human cardiac stem cells with reduced notch signaling show enhanced therapeutic potential in a rat acute infarction model
Matsuda T, et al.
Circulation Journal, 78(1), 222-231 (2014)
Immunoimaging of CXCR4 expression in brain tumor xenografts using SPECT/CT
Nimmagadda S, et al.
Journal of Nuclear Medicine, 50(7), 1124-1130 (2009)

Questions

1–5 of 5 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. Can one use Product C5914, Cell Dissociation Solution, with tissue?

    1 answer
    1. We have only used with monolayer cell culture, and therefore we have no information on its usage with tissue.

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  3. Does Product C5914, Cell Dissociation Solution, work with epithelial cells?

    1 answer
    1. It will work with most cell types.

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  4. Does Product C5914, Cell Dissociation Solution, contain EDTA?

    1 answer
    1. Yes, it does contain EDTA; the concentration is proprietary.

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  5. When trypsin is used to detach cells, one usually adds fetal bovine serum (FBS) to the media. Should FBS be used with Product C5914, Cell Dissociation Solution?

    1 answer
    1. No. FBS is used to inactivate trypsin. This product is not enzymatic, so there is no need to use FBS. The cells can be washed with phosphate buffered saline (PBS). When the cells are to be cultured, then one would need to add FBS.

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