The reconstituted product is stable for 1 month at –20 °C and for at least 2 months at –70 °C. Product 528205, a prepared solution, also stored at -70°C, is assigned a retest schedule of every two years. See the link below to review this product page:
https://www.sigmaaldrich.com/US/en/product/mm/528205
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产品名称
血纤维蛋白溶酶原激活物抑制剂1(PAI-1) 人, recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
重组
expressed in E. coli
质量水平
方案
≥90% (SDS-PAGE)
表单
lyophilized powder
比活
≥200,000 units/mg protein
分子量
~43 kDa
溶解性
water: 0.1 mL, clear, colorless
UniProt登记号
运输
dry ice
储存温度
−20°C
基因信息
human ... SERPINE1(5054)
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一般描述
应用
生化/生理作用
纤溶酶原激活物抑制剂1（PAI-1）是丝氨酸蛋白酶抑制剂Serpin超家族的一员，可参与到细胞外基质重塑并涉及血管生成、趋化性、排卵和胚胎生成等过程。
纤溶酶原激活物抑制剂1（PAI-1）蛋白属于丝氨酸蛋白酶抑制剂的Serpin超家族。它是组织纤溶酶原激活物（tPA）和尿激酶（uPA）的主要抑制剂，并也是纤溶酶原的激活剂，因此可抑制纤维蛋白溶解过程。 PAI可参与到细胞外基质的重塑，并涉及血管生成、趋化性、排卵和胚胎生成等过程。 PAI-1在各种疾病状态中的水平都有所上升，如多种癌症形式、肥胖症和代谢综合征。
单位定义
1单位可在生理状态下（中性pH和37 ºC）不可逆的抑制（100%抑制）1单位的组织纤溶酶原激活物（tPA）。
外形
以含有0.5 M氯化钠、20 mM 乙酸钠和50 mg/mL 海藻糖的一种溶液冻干粉形式提供。
重悬
用超纯水重悬成0.25 mg/ml。在0.5 M氯化钠、20 mM 乙酸钠和50 mg/mL 海藻糖中，含重悬产物0.25 mgP/mL。
分析说明
该产品是一种活性人PAI-1蛋白，分子量为43 kDa。
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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Cutaneous tissue injury, both in vivo and in vitro, initiates activation of a "wound repair" transcriptional program. One such highly induced gene encodes plasminogen activator inhibitor type-1 (PAI-1, SERPINE1). PAI-1-GFP, expressed as a fusion protein under inducible control of +800
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Once it has been reconstituted, what is the recommended storage temperature? Will the reconstituted liquid be stable at 4 C for short-term storage (e.g. <24 hrs)?
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