This product has an optimum pH range of 5–9 and will maintain 98% of activity after 60 minutes at pH 6.2 at 85°C. This material is tested pH 6.9 at boiling temperature for 15 minutes. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/297/885/a3403pis.pdf
A3403
α-淀粉酶 来源于地衣芽孢杆菌
Type XII-A, saline solution, ≥500 units/mg protein (biuret)
别名:
Termamyl® 2X, 1,4-α-D-葡聚糖-葡萄糖水解酶
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About This Item
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类型
Type XII-A
表单
saline solution
比活
≥500 units/mg protein (biuret)
分子量
57.6 kDa
环保替代产品特性
Waste Prevention
Design for Energy Efficiency
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sustainability
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环保替代产品分类
储存温度
2-8°C
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一般描述
应用
生化/生理作用
注意
单位定义
外形
法律信息
警示用语:
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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实验方案
可根据此操作规程测定α-淀粉酶的活性。该α-淀粉酶酶促测定将会指导您完成整个过程及必要的计算。
Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.
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What is the optimal conditions (pH and temperature) for this enzyme to degrade starch? Will it deactivate after heating to 100C?
1 answer-
Helpful?
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Dear Sir or Madam Could you tell me exactly in what solution the a-amylase is provided? Unfortunately only a "saline sucrose solution" is stated. I would need the exact composition in order to prepare a negative control in my assay. kind regards
1 answer-
All that we can disclose is that this product is supplied as an aqueous solution containing sodium chloride and sucrose with >=10 mg/mL protein.
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