The specific salmon species is not determined for this product. See the link below to review a sample Certificate of Origin for this material:
https://www.sigmaaldrich.com/coo/undefined/D9156/SLBM7249V.
选择尺寸
1 ML
$132.00
5 ML
$618.00
5 X 1 ML
$705.00
$132.00
请联系客服了解存货情况
新价格，新优惠！
所有图片(1)
选择尺寸
变更视图
1 ML
$132.00
5 ML
$618.00
5 X 1 ML
$705.00
About This Item
$132.00
请联系客服了解存货情况
新价格，新优惠！
推荐产品
生物来源
fish testis (salmon)
质量水平
等级
for molecular biology
方案
9-11 mg/mL (DNA concentration)
表单
solution
浓度
10 mg/mL in H2O
运输
dry ice
储存温度
−20°C
正在寻找类似产品? 访问 产品对比指南
一般描述
是从鲑鱼睾丸分离优质单链模板DNA的即用型解决方案。 溶液的供应浓度为9-12 mg/ml。
应用
许多因素有助于提高核酸杂交的信噪比。 这些因素包括溶剂（甲酰胺）的存在、杂交温度、杂交长度、杂交溶液体积、搅拌程度和方法、封闭剂的使用、探针浓度和比活性、使用分子试剂提高核酸重缔合的速率以及在洗涤膜的严格程度。
为了减少探针与基质的任何非特异性杂交，必须使用封闭剂。 通常，使用封闭试剂、去污剂以及变性片段化DNA的组合来实现此目的。 Sigma提供来自多种物种的超声处理的变性DNA，可用作Northern和Southern印迹以及其他核酸杂交技术的封闭剂。
为了减少探针与基质的任何非特异性杂交，必须使用封闭剂。 通常，使用封闭试剂、去污剂以及变性片段化DNA的组合来实现此目的。 Sigma提供来自多种物种的超声处理的变性DNA，可用作Northern和Southern印迹以及其他核酸杂交技术的封闭剂。
适合用作Northern和Southern印迹的封闭剂。
制备说明
将该DNA用乙醇沉淀并超声处理，产生单链片段，其与587和831碱基对标记片段共迁移。
其他说明
DNA溶液要在室温下放置进行再退火，因此建议使用前将溶液煮沸10分钟，然后在冰上至少冷却5分钟。
相关产品
产品编号
说明
价格
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
H W Ziegler-Heitbrock et al.
Blood, 79(2), 503-511 (1992-01-15)
Cytokine expression was analyzed in CD14++ regular monocytes and in the novel subset of CD14+/CD16+ small monocytes. Biologic activity for tumor necrosis factor (TNF), interleukin-1 (IL-1), and IL-6 in the supernatant of elutriator-enriched, cell sorter-purified small monocytes was about 10-fold
J W Said et al.
Blood, 90(11), 4278-4282 (1997-12-31)
We have recently demonstrated the presence of Kaposi's sarcoma-associated herpesvirus (KSHV) in cultured bone marrow (BM) stromal dendritic cells from all patients with myeloma studied. To show that these findings were not an artifact of tissue culture, we performed in
Marion Herbette et al.
PLoS genetics, 17(7), e1009662-e1009662 (2021-07-07)
Segregation Distorter (SD) is a male meiotic drive system in Drosophila melanogaster. Males heterozygous for a selfish SD chromosome rarely transmit the homologous SD+ chromosome. It is well established that distortion results from an interaction between Sd, the primary distorting
Maarten Holkers et al.
Nature methods, 11(10), 1051-1057 (2014-08-26)
Engineered sequence-specific nucleases and donor DNA templates can be customized to edit mammalian genomes via the homologous recombination (HR) pathway. Here we report that the nature of the donor DNA greatly affects the specificity and accuracy of the editing process
Manolis Kellis et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(17), 6131-6138 (2014-04-23)
With the completion of the human genome sequence, attention turned to identifying and annotating its functional DNA elements. As a complement to genetic and comparative genomics approaches, the Encyclopedia of DNA Elements Project was launched to contribute maps of RNA
商品
Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.
转化是将外源DNA引入细胞，引起遗传变异或基因修饰的过程。1928年，Griffith首次报道了利用肺炎链球菌进行的转化。Avery等人于1944年证明了DNA转化的原理。
实验方案
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.
酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
-
What Salmon sp. D9156 is prepared from?
1 answer-
Helpful?
-
Active Filters
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门
